Abstract
The continuous flow method was used to study the decomposition of uniformly tagged glucose in soil with different inorganic nitrogen and phosphorus levels. It was found that the amount of glucose carbon mineralized to carbon dioxide was higher if nitrogen and phosphorus were added together with the glucose. Some of the labelled carbon escaped from the soil and the amount of leached-out carbon was in inverse proportion to the amount of nitrogen and phosphorus in the soil. The level of mineral nutrient elements stimulated the rate of glucose mineralization in the initial phase of the continuous process. The rate of glucose mineralization in the steady state was stimulated in soil continuously enriched with glucose together with nitrogen and phosphorus. The quantitative relationship between the assimilation and oxidation of glucose carbon depended on the nitrogen and phosphorus concentration and was in inverse proportion to the mineral element level. The continuous addition of glucose stimulated decomposition of the native soil organic matter. The resultant priming effect was balanced, however, by the retention of glucose carbon in the soil, with the result that the carbon balance remained positive. The rate of glucose oxidation, the amount of carbon retained in the soil and the priming effect of glucose were strongly influenced by the flow rate.
Abstract
Непрерывный проточный метог был использован для изучения разложения в почве единообразно меченой глюкозы при различных уровнях неорганического азота и фосфора. Было установлено, что количество глюкозы, меченой14C и окисляемой до двуокиси углерода, находилось в прямой зависимости от содержания азота и фосфора. Значительная часть14C уходила из почвы, и количество бымываемого14C было обратно пропорционально содержанию в почве азота и фосфора. Согержание элементов минерального питания влияло на скорость минерализации глюкозы в начальной фазе непрерывного процесса, а отчасти также на скорость минерализации глюкозы в состоянии равновесия. Отношение между ассимиляцией и окислением глюкозы-14C зависело от содержания азота и фосфора и понижалось при повышении содержания минеральных элементов. Непрерывное поступление глюкозы стимулировало разложение нативного органического вещества почвы. Но результирующий priming effect перекрывался задержкой14C в почве, так что углеродныи баланс оставался положительным. Скорость протока существенно влияла на скорость окисления глюкозы, количество14C, задерживающегося в почве, и на priming effect глюкозы.
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References
Barrow, N. J.:The effect of varying the nitrogen, sulphur and phosphorus content of organic matter on its decomposition. Austral. J. Agric. Res. 11: 317, 1960.
Bingeman, C. W., Varner, J. E., Martin, W. P.:The effect of the addition of organic materials on the decomposition of an organic soil. Soil Sci. Amer. Proc. 17: 34, 1953.
Broadbent, F. E.:Nitrogen release and carbon loss from soil organic matter during decomposition of added plant residues. Soil Sci. Soc. Amer. Proc. 13: 246, 1948.
Broadbent, F. E., Bartholomew, W. V.:The effect of the quantity of plant material added to soil on its rate of decomposition. Soil Sci. Soc. Amer. Proc. 13: 271, 1948.
Busch, A. W., Myrick, N.:Aerobic bacterial degradation of glucose. Journal WPCF 33: 897, 1961.
Clifton, C. E.:Microbial assimilations. Adv. Enzymol. 6: 269, 1946.
Clifton, C. E.:Oxidative assimilation and distribution of glucose in Bacillus cereus. J. Bacteriol. 83: 66, 1962.
Drobník, J.:Primary oxidation of organic matter in the soil. I. The form of respiration curves with glucose as the substrate. Plant & Soil 12: 199, 1960.
Hallam, M. J., Bartholomew, M. V.:Influence of rate of plant residue addition in accelerating the decomposition of soil organic matter. Soil Sci. Soc. Amer. Proc. 17: 365, 1953.
Jansson, S. L.:Tracer studies on nitrogen transformations in soil with special attention to mineralization-immobilization relationships. Kungl. Lantbrukshögskolands Annaler 24: 101, 1958.
Jansson, S. L.:On the establishment and use of tagged microbial tissue in soil organic matter research. Trans. 7th Intern. Congr. Soil Sci. 2: 635, 1960.
Lees, H., Porteous, J. W.:The release of carbon dioxide from soils percolated with various organic materials. Plant & Soil 2: 231, 1950.
Macura, J.:Continuous flow method in soil microbiology. I. Apparatus. Fol. microbiol. 6: 328, 1961.
Macura, J., Kune, F.:Continuous flow method in soil microbiology. II. Observations on glucose metabolism. Fol. microbiol. 6: 398, 1961.
Macura, J., Málek, I.:Continuous flow method for the study of microbiological processes in soil samples. Nature 182: 1796, 1958.
Macura, J., Szolnoki, J., Vančura, V.:Decomposition of glucose in soil. Soil Organisms. Proc. of the Colloquium on Soil Faune, Soil Microflora and their Relationships, pp. 231. Ed.: J. Doeksen & J. van der Drift. Amsterdam 1963.
Magasanik, B., Neidhardt, F. C., Levin, A. P.:Metabolic regulation of enzyme synthesis in bacteria. Physiological adaptation (Ed.: Prosser) pp. 159, American Physiological Society, Washington 1958.
Mayaudon, J., Simonart, P.:Étude de la décomposition de la matière organique dans le sol au moyen de cerbon radioactif. II. Décomposition du glucose radioactif dans le sol. A. Répartition de la radioactivité dans les fractions humique du sol. Plant & Soil 9: 375, 1958.
Mortensen, J. L.:Decomposition of organic matter and mineralization of nitrogen in Brookston silt loam and alfalfa green manure. Plant & Soil 19: 374, 1963.
Porges, N., Jasewicz, L., Hoover, S. R.:Principles of biological oxidation. In “Biological Treatment of Sewage and Industrial Wastes”. Reinhold Publ. Corp., New York 1956.
Simonart, P., Mayaudon, J.:Étude de la décomposition de la matière organique dans le sol, au moyen de carbonne radioactif. I. Cinétique de l'Oxydqtion en CO 2 de divers substrats radioactifs. Plant & Soil 9: 367, 1958a.
Simonart, P., Mayaudon, J.:Étude de la décomposition de la matière organique dans le sol au moyen de carbon radioactif. II. Décomposition du glucose radioactif dans le sol. B. Répartition de la radioactivité dans 1′α-humus. Plant & Soil 9: 381, 1958b.
Somogyi, M.:Notes on sugar determination. J. biol. Chem. 195: 19, 1952.
Sørensen, H.:Studies on the decomposition of C 14-labelled barley straw in soil. Soil Sci. 95: 45, 1963.
Stotzky, G., Mortensen, J. L.:Effect of addition level and maturity of rye tissue on the decomposition of a muck soil. Soil Sci. Soc. Amer. Proc. 22: 521, 1958.
Stotzky, G., Norman, A. G.:Factors limiting microbial activities in soil. I. The level of substrate, nitrogen and phosphorus. Arch. Mikrobiol. 40: 341, 1961.
Szolnoki, J., Vágó, É. T.:Abbau und Humifikation von mit dem Isotop C 14 markierten Stroh in Boden. Acta Agron. 9: 407, 1959.
Vančura, V., Macura, J., Szolnoki, J.:Products of glucose metabolism in the soil. 8th Intern. Congr. Soil. Sci., Bucurest 1964.
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Macura, J., Szolnoki, J., Kunc, F. et al. Decomposition of glucose continuously added to soil. Folia Microbiol 10, 44–54 (1965). https://doi.org/10.1007/BF02869840
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DOI: https://doi.org/10.1007/BF02869840