Abstract
Lycopersicon esculentum cv. Linia XXIV (L) and cv. Ostravské (O) was infected withAgrobacterium tumefaciens T37 (pTiT37). 37 L tumors and 33 O tumors were isolated. 13.5 % L tumors and 3.0 % O tumors regenerated shoots producing nopaline synthase. The shoots formed roots after transfer on R3B medium without phytohormones. From 10 primary shoots 35 plants cultivated in an unsterile soil were obtained by cutting. 6 selfpollinated plants did not produce any fruits. Fruits obtained through backcrossing had dramatically reduced seed numbers. The same is true of some other transformed plants with nearly normal pollen. Most transformed plants were susceptible to superinfection withA. tumefaciens T37 and B6-806. The incorporation of T-DNA into plant genome seems to cause not only male sterility, but also some kind of female sterility.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barton, K. A., Binns, A. N., Matzke, A. J. M., Chilton, M.-D.: Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny. - Cell32: 1033–1043, 1983.
Buchmann, J., Marner, F. J., Schröder, G., Waffenschmidt, S., Schröder, J.: Tumour genes in plants: T-DNA encoded cytokinin biosynthesis. - EMBO J.4: 843- 859, 1985.
Chareonboonsit, S., Splittstoesser, W. E., George, W. L.: The effect of pollination methods and auxin applications upon parthenocarpic fruit set and development in tomato. - Scientia Hort.27: 1–8, 1985.
Chilton, M.-D., Drummond, M. H., Merlo, D. J., Sciaky, D., Montoya, A. L., Gordon, M. P., Nester, E. W.: Stable incorporation of plasmid DNA into higher plant cells: The molecular basis of crown gall tumorigenesis. - Cell11: 263–272, 1977.
Chyi, Y.-S., Jorgensen, R. A., Goldstein, D., Tanksley, S. D., Loaiza-Figueroa, F.: Locations and stability ofAgrobacterium-mediated T-DNA insertion in theLycopersicon genome. - Mol. gen. Genet.204: 64–69, 1986.
De Cleene, M., DeLey, J.: The host range of crown gall. - Bot. Rev.42: 389–466, 1976.
Gabara, B., Kubicki, B.: Factors affecting the production of seeds in fully fertile tomatoes (Lycopersicon esculentum Mill.) and those showing a tendency to parthenocarpy. - Acta Soc. Bot. Pol.54: 223–229, 1985.
George, W. L., Scott, J. W., Splittstoesser, W. E.: Parthenocarpy in tomato. - Hort. Rev.6: 65–84, 1984.
Hain, R., Stabel, P., Czernilofsky, A. R., Steinbiss, H. H., Herrera-Estrella, L., Schell, J.: Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts. - Mol. gen. Genet.199: 161–168, 1985.
Kahl, G., Schell, J. S.: Molecular Biology of Plant Tumors. - Academic Press, Paris - San Diego - San Francisco - São Paulo - Sydney - Tokyo - Toronto 1982.
Koornneef, M., Hanhart, C., Jongsma, M., Toma, I., Weide, R., Zabel, P., Hille, J.: Breeding of a tomato genotype readilly accessible to genetic manipulation. - Plant Sci.45: 201–208, 1986.
Langley, L. A., Kado, C. I.: Studies inAgrobacterium tumefaciens. Conditions for mutagenesis by N-metyl-N′-nitro-N-nitrosoguanidine and relationshipof A. tumefaciens mutants to crowngall tumor induction. - Mutat. Res.14: 277–286, 1972.
Lin, S., Splittstoesser, W. E., George, W. L.: A comparison of normal seeds and pseudoembryos produced in parthenocarpic fruit of “Severianin” tomato. - Hort. Sci.18: 75–76, 1986.
Memelink, J., Wullems, G. J., Schilperoort, R. A.: Nopaline T-DNA is maintained during regeneration and generative propagation of transformed tobacco plants. - Mol. gen. Genet.190: 516–522, 1983.
Meredith, C. P.: Shoot development in established callus cultures of cultivated tomato (Lycopersicon esculentum Mill.). - Z. Pflanzenphysiol.95: 405–411, 1979.
NečÁsek, J., DusbÁbkovÁ, J., VlasÁk, J.: Bacteriocin production inAgrobacterium tumefaciens. - Folia microbiol.30: 353–358, 1985.
Negrutiu, J., Beeftinck, F., Jacobs, M.:Arabidopsis thaliana as a model system in somatic cell genetics. I. Cell and tissue culture. - Plant Sci. Lett.5: 293–304, 1975.
Nester, E. W., Gordon, M. P., Amasino, R. M., Yanofsky, M. F.: Crown gall: a molecular and physiological analysis. - Annu. Rev. Plant Physiol.35: 387–413, 1984.
Norton, R. A., Towers, G. H. N.: Transmission of nopaline crown gall tumor markers through meiosis in regenerated whole plants ofBidens alba. - Can. J. Bot.62: 408–413, 1984.
Ooms, G., Bains, A., Burrell, M., Karp, A., Twell, D., Wilcox, E.: Genetic manipulation in cultivars of oilseed rape (Brassica napus) usingAgrobacterium. - Theor. appl. Genet.71: 325–329, 1985.
Otten, L., de Greve, H., Hernalsteens, J. P., van Montagu, M., Schieder, O., Straub, J., Schell, J.: Mendelian transmission of genes introduced into plants by Ti plasmid ofAgrobacterium tumefaciens. - Mol. gen. Genet.183: 209–213, 1981.
Otten, L., Schilperoort, R. A.: A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities. - Biochim. biophys. Acta527: 497–500, 1978.
PavingerovÁ, D., Ondřej, M., Matoušek, J.: Analysis of progeny ofArabidopsis thaliana plants regenerated from crown gall tumors. - Z. Pflanzenphysiol.112: 427–433, 1983.
Picken, A. J. F.: A review of pollination and fruit set in the tomato (Lycopersicon esculentum Mill.). - J. hort. Sci.59: 1–13, 1984.
Potrykus, I., Paszkowski, J., Saul, M. W., Petruska, J., Shillito, R. D.: Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer. - Mol. gen. Genet.199: 169–177, 1985.
Pühler, A. (ed.): Molecular Genetics of the Bacteria-Plant Interactions. - Springer-Verlag, Berlin-Heidelberg-New York-Tokyo 1983.
Schell, J., Van Montagu, M., Holsters, M., Zambryski, P., Joos, H., Herrera-Estrella, L., Depicker, A., Hernalsteens, J.-P., De Greve, H., Willmitzer, L., Schröder, J.: Ti plasmids as gene vectors for plants. - In: Kumar, A. (ed.): Eukaryotic Gene Expression. Pp. 141–160. Plenum Publ. Corp., London 1984.
Spano, L., Costantino, P.: Regeneration of plants from callus cultures of roots induced byAgrobacterium rhizogenes in tobacco. - Z. Pflanzenphysiol.106: 87–92, 1982.
Tepfer, D.: The biology of genetic transformation of higher plants byAgrobacterium rhizogenes. - In: Pühler, A. (ed.): Molecular Genetics of the Bacteria-Plant interactions. Pp. 248–258. Springer-Verlag, Berlin - Heidelberg - New York- Tokyo 1983.
Thomashow, M. F., Hugly, S., Buchholz, W. G., Thomashow, L.: Molecular basis for the auxin-independent phenotype of crown gall tumor tissues. - Science231: 616–618, 1986.
TroníčkovÁ, E.: Selfsterile forms as applied in hybrid-seed production of various crops. - Genet. Siecht. (Praha)9 (46): 153–154, 1973.
Wullems, G. J., Molendijk, L., Ooms, G., Schilpeboort, R. A.: Retention of tumour markers in F1 progeny plants from in vitro induced octopine and nopaline tumour tissues. - Cell24: 719–727, 1981.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
NečÁsek, J., DusbÁbkovÁ, J. & PekÁrkovÁ-TroníčkovÁ, E. Whole plants regeneration from crown galls ofLycopersicon esculentum . Biol Plant 30, 1–8 (1988). https://doi.org/10.1007/BF02876415
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02876415