Abstract
The role of the “antibody cofactor” and of other heat-labile serum components (complement) in the neutralization of the ϕX 174 bacteriophage by means of specific antibodies was studied. Sera of white mice, guinea-pigs and rabbits obtained mainly early after phage administration were investigated. The character of antibodies was estimated from their sensitivity to 2-mercaptoethanol or else by chromatography on Sephadex G-200. Sera from the first days after the administration of the phage containing mostly type 19S antibodies, and sera from later periods after the administration containing mostly type 7S antibodies, were tested. (Some evidence was also obtained about the formation of slowly sedimenting antibodies sensitive to 2-mercaptoethanol in the rabbit.) With a single exception the tested sera showed no significant decrease of the neutralization activity after 30-mins. heating at 56°C or at 60°C and no increase of the neutralization power could be observed after the application of homologous or normal mouse serum. It is concluded that the heat-labile components of normal sera including the complement and the “antibody cofactor” play no role in specific phage neutralization.
Abstract
Изучалась возможная роль «кофактора антител», а также других термолабильных сывороточных компонентов (комплемент), при нейтрализации бактериофага ΦX174 специфическими антителами. Исследовались сыворотки белых мышей, морских свинок и кроликов, полученные, главным образом, на ранних сроках после введения фага. Характер антител определяли по чувствительности к 2-меркаптоэтанолу, а иногда также с помощью хроматографии на Sephadex G-200. Тестировались сыворотки, полученные в первые дни после применения фага, преимущественно содержавшие антитела типа 19S, и сыворотки, полученные в более поздний период, с более значительным содержанием антител 7S. (Были получены также сведения относительно образования медленно осаждающихся антител, чувствительных к 2-меркаптоэтанолу, у кролика.) За одним исключением, в исследуемых сыворотках не наблюдалось ни достоверного понижения активности нейтрализации после их 30-минутного прогревания при 56°C или 60°C, ни потенцирования нейтрализации после прибавления гомологичной (или мышиной) сыворотки.—Делается вывод, что термолабильные компоненты нормальных сывороток, включая комплемент и «кофактор антител», не играют роли в специфической нейтрализации фага.
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Styk, B., Závada, J. & Hána, L. The role of nonspecific serum factors in the antibody response to the ΦX 174 bacteriophage. Folia Microbiol 9, 263–268 (1964). https://doi.org/10.1007/BF02873304
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DOI: https://doi.org/10.1007/BF02873304