Abstract
Wall-less protoplasts of the moldRhizopus nigricans were obtained by means of enzymic digestion of the walls of vegetative hyphae by Helix pomatia digestive juice. After removing the enzymes the protoplasts transferred to a nutrition medium regenerate. In the first stage of this process the protoplasts from a firm wall and then the hyphae begin to grow. Regeneration results in the formation of a normal mycelium. If in the course of regeneration of the protoplasts snail enzymes are present, they partially block the synthesis of the wall and the protoplasts transform into incessantly growing giant formations. Re-establishment of morphogenesis begins only after eliminating the effect of the snail enzymes.
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Gabriel, M. Formation and regeneration of protoplasts in the moldRhizopus nigricans . Folia Microbiol 13, 231–234 (1968). https://doi.org/10.1007/BF02871039
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DOI: https://doi.org/10.1007/BF02871039