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Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy

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Abstract

The 60% ethanolic extract fromGracilaria textorii (Rhodophyta) was degraded with β-agarase, and certain charged (sulfated) and neutral oligosaccharides were separated by using DEAE Sephadex A25 and Bio- gel P6, P2 chromatographic techniques. Some of the charged oligomers were verified to be neoagarotetraose-63-sulfate (DP2), neoagarohexaose-63, 65-disulfate (DP3) and neoagarooctaose-63, 65, 67-trisulfate (DP4) by using13C- and1H-NMR spectroscopy. One neutral oligomer was assumed to be a mixture of methylated neoagarotetraoses (DP2) by1H-NMR spectroscopy.

These oligomers assigned by their chemical shifts may be used as the model compounds for the structural investigation of the agar-type sulfated polysaccharides using the β-agarase degradation method.

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Author notes

  1. M. Lahaye

    Present address: INRA-LBTG, B. P. 527, 44026, Nantes Cedex, France

Authors and Affiliations

  1. Institute of Oceanology, Academia Sinica, Qingdao

    Ji Minghou

  2. Department of Microbiology and Immunology, McGill University, Montreal, Canada

    M. Lahaye & W. Yaphe

Authors
  1. Ji Minghou
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  2. M. Lahaye
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  3. W. Yaphe
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Contribution No. 1749 from the Institute of Oceanology, Academia Sinica.

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Minghou, J., Lahaye, M. & Yaphe, W. Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy. Chin. J. Ocean. Limnol. 8, 135–149 (1990). https://doi.org/10.1007/BF02850446

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  • DOI: https://doi.org/10.1007/BF02850446

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