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Structural studies on agar fractions extracted sequentially from chinese red sea weeds:Gracilaria sjeostedtii, G. textorii andG. salicornia using13C-NMR and IR spectroscopy

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Abstract

The polysaccharides of three species of ChineseGracilaria (Rhodophyta) were extracted by sequential solvent extraction and the chemical structures of the fractions obtained were investigated by using13C-NMR and IR spectroscopy.

The13C-NMR spectra showed that the main extract, cold water fraction, fromG. sjeostedtii consisted of repeating disaccharides of agarobiose and (1→3)-β-D-galactopyranosy 1-(1→4)-α-L-galactopyranose 6-sulfate, the latter being a precursor to agarobiose and converted to agarobiose after alkali modification. The component of the 60% ethanol fraction fromG. textorii was composed mainly of agarose 6-sulfate, 6-O-methyl-agarose and some amount of agarose, whse structures remained unchanged after alkali treatment. The autoclave extract fromG. salicornia revealed a complex structure composed of 6-O-methyl-agarose, 2-O-methyl-agarose, branched 4-O-methyl-L-galactose and agarose 4-sulfate. All the substituents in these agaroses were stable against alkali treatment. The alkali extracts fromG. sjeostedtii andG. textorii showed the distinct spectra of amylopectin-like floridean starch, while that fromG. salicornia showed a mixture of agaroses and floridean starch.

From the IR spectra the relative contents of total sulfate, 3,6-anhydro-galactose, 4-sulfate and 6-sulfate in some fractions of polysaccharides of the algae were qualitatively estimated by the ratios of absorption bands of 1250/2920, 930/2920, 850/2920 and 820/2920, respectively.

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Authors and Affiliations

  1. Institute of Oceanology, Academia Sinica, Qingdao

    Ji Minghou

  2. Dept of Microbiology & Immunology, McGill University, Montreal, Canada

    M. Lahaye & W. Yaphe

Authors
  1. Ji Minghou
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  2. M. Lahaye
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  3. W. Yaphe
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Contribution No. 1489 from Institute of Oceanology, Academia Sinica

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Minghou, J., Lahaye, M. & Yaphe, W. Structural studies on agar fractions extracted sequentially from chinese red sea weeds:Gracilaria sjeostedtii, G. textorii andG. salicornia using13C-NMR and IR spectroscopy. Chin. J. Ocean. Limnol. 6, 87–103 (1988). https://doi.org/10.1007/BF02847829

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  • DOI: https://doi.org/10.1007/BF02847829

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