Abstract
Root biomass of rice seedlings was increased at lower concentration of exogenous NH +4 , but it was decreased at higher concentration of exogenous NH +4 . The level of free NH +4 in the roots was accumulated gradually with the increase of NH +4 concentration in the nutrient solution. The content of the soluble proteins was essentially constant at higher NH +4 . The activities of glutamine synthetase (GS), NADH-dependent glutamate synthase (NADH-GOGAT), and NADH-dependent glutamate dehydrogenase (NADH-GDH) were risen with exogenous NH +4 concentration at the lower NH +4 concentration range. But the activities of GS and NADH-GOGAT were declined, and the level of NADH-GDH activity was kept constant under higher NH +4 concentration. The GS/GDH ratio suggested that NH +4 was assimilated by GS-GOGAT cycle under lower NH +4 concentration, but NADH-GDH was more important for NH +4 assimilation and detoxifying NH +4 to the tissue cells at the higher NH +4 level. According to the growth and the activity changes of these ammonium-assimilating enzymes of rice seedling roots, 10. 0 μg/mL NH +4 -N in nutrient solution was more suitable to the rice growth.
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Foundation item: Supported by the National Natural Science Foundation of China (No. 3987052), Natural Science Foundation of Hubei Province and International Rice Research Institute, P.O. Box. 3217, 1271 Makati City, Philippines.
Biography: LI Ze-song(1968-), male, Graduate student.
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Ze-song, L., Chu-fu, Z., Qing-hua, L. et al. Effect of exogenous ammonium on glutamine synthetase, glutamate synthase, and glutamate dehydrogenase in the root of rice seedling. Wuhan Univ. J. Nat. Sci. 4, 358–362 (1999). https://doi.org/10.1007/BF02842373
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DOI: https://doi.org/10.1007/BF02842373