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Modified protocols for rapid carrot genomic DNA extraction and AFLP™ analysis using silver stain or radioisotopes

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Abstract

AFLP™ is one of the most frequently used techniques for identification of molecular markers. We have modified the procedures for genomic DNA extraction, AFLP product generation and silver staining in order to speed up analyses and screen large numbers of plant samples. Using this protocol, we were able to achieve an 82% reduction of costs without compromising the reliability and quality of data gathered.

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Author notes

  1. M. Briard

    Present address: Institut National d’Horticulture, 2 Rue Le Nôtre, 49045, Angers Cedex 01, France

Authors and Affiliations

  1. Institut National d’Horticulture, 2 Rue Le Nôtre, 49045, Angers Cedex 01, France

    V. Le Clerc

  2. Krakow Agricultural University, Krakow, Poland

    D. Grzebelus

  3. USDA, Agricultural Research Service, University of Wisconsin, Madison, WI, USA

    D. Senalik & P. W. Simon

Authors
  1. M. Briard
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  2. V. Le Clerc
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  3. D. Grzebelus
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  4. D. Senalik
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  5. P. W. Simon
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Corresponding author

Correspondence to M. Briard.

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Briard, M., Le Clerc, V., Grzebelus, D. et al. Modified protocols for rapid carrot genomic DNA extraction and AFLP™ analysis using silver stain or radioisotopes. Plant Mol Biol Rep 18, 235–241 (2000). https://doi.org/10.1007/BF02823994

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  • DOI: https://doi.org/10.1007/BF02823994

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