Abstract
Commercial roasted salted peanuts (3% NaCl), popcorn (1% NaCl), summer-squash (9% NaCl), sunflower (3% NaCl) and wild-melon (3% NaCl) seeds are polluted with fungi, mostlyAspergillus flavus, A. niger, Penicillium chrysogenum, P. corylophilum andRhizopus stolonifer. Contamination of popcorn with the fungi is about 10 times higher than in the other foods. These fungi, common also on unsalted seeds, are significantly inhibited in seeds (30% moisture content) treated with 9–21% NaCl. The halotolerantA. wentii represents the main fungus recovered from seeds treated by 15–21% NaCl. 9% NaCl stimulated emodin production byA. wentii on peanut and citrinin production byP. chrysogenum on popcorn and sunflower. Aflatoxin, citrinin and emodin production on popcorn persisted up to 15% NaCl. Popcorn is thus strongly susceptible to fungal invasion and toxin pollution. The halotolerance ofA. wentii was confirmed by its strong permanent growth in liquid medium at up to 15% NaCl. At 3% NaCl the mycelial growth and nitrogen content increased while the level of emodin and lipid production decreased. CO2 evolution strongly increased at 9–15% NaCl as a characteristic ofA. wentii salt tolerance. Emodin inhibited seed viability and the inhibition dose for 50% reduction (LD50) was 65 mg/L for popcorn and 45 mg/L for sunflower.
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Arai M., Hibino T.:Cancer Lett.17, 281–287 in C.F. Jelinek, A.E. Pohland, G.E. Wood (Eds):Review of Mycotoxin Contamination. J. Assoc. Off. Analyt. Chem.72, 223–230 (1989).
Bankole S.A.: Moisture content, mould invasion and seed germinability of stored melon.Mycopathologia122, 123–126 (1993).
Bates L.S., Waldes R.P., Teare I.D.: Rapid determination of free proline for water stress studies.Plant & Soil39, 205–207 (1973).
Bendele S.A., Garlton W.W., Krogh O., Lillehoj E.B.: Ochratoxin A carcinogenesis in mouse. Abstr. no. 21, 3rd Mycological Congress, Tokyo 1983.
Booth C.:Fusarium Laboratory Guide to the Identification of the Major Species. Commonwealth Mycological Institute, Kew, Surrey (England) 1977.
Christensen M., Raper K.P.: Synoptic key toAspergillus nidulans group species andEmericella species.Trans. Brit. Mycol. Soc.71, 177–191 (1978).
Ciegler A.: Mycotoxin. Occurrence, chemistry, biological activity.Lloydia38, 21–35 (1975).
Ciegler A., Vesconder R.F., Jackion L.K.: Production and biological activity of patulin and citrinin fromPenicillium expansum.Appl. Environ. Microbiol.33, 1004 (1977).
Coupland K., Niehaus W.G.: Effect of nitrogen supply, Zn and salt concentration on kojic acid and versicolorin biosynthesis byAspergillus parasiticus.Exp. Mycol.11, 206–213 (1987).
Damodaran C., Ramadoss S.S., Shanmugasundaram E.R.B.: A rapid procedure for the isolation, identification and estimation of citrinin.Anal. Biochem.52, 482 (1973).
De Gier J., Van Echteld C.J.A., De Kruiff B.: Lipid organisation and barrier functions of membranes. pp. 315–325 in J.F.G.M. Wintermans, P.G.C. Kuiper (Eds):Biochemistry and Metabolism of Plant Lipids. Elsevier, Amsterdam 1982.
De-Vries G.A.:Contribution to Knowledge of the Genus Cladosporium Link ex Fr. Vitgeverij and Drukkerij. Hollandia Press, Baarn 1952.
El-Maghraby O.M.O., El-Maraghy S.S.: Mycoflora and mycotoxins of peanut (Arachis hypogaea L.) seeds in Egypt: sugar fungi and natural occurrence of mycotoxins.Mycopathologia98, 165–170 (1987).
Hasan H.A.H.: Selective effect of some pesticides on mycoflora and mycotoxins production in corn grains and sunflower seeds.MSc Thesis. Faculty of Science, Assiut University (Egypt) 1988.
Katta S.K., Bullerman L.B.: Effect of high temperature and relative humidity on mold content and quality of stored popcorn.J. Food Prot.58, 1018–1022 (1995).
Katta S.K., Eskridge K.M., Bullerman L.B.: Mold content of commercial popcorn.J. Food Prot.58, 1014–1017 (1995).
Kyo Sato: Relations between soil microflora and CO2 evolution upon decomposition of cellulase.Plant & Soil161, 251–258 (1981).
Lea T., Steien K., Stormer F.C.: Mechanism of ochratoxin A-induced immunosuppression.Mycopathologia107, 153–159 (1989).
Lee L.S., Dunn J.J., Delucca A.J., Ciegler A.: Role of lactone ring of aflatoxin B1 in toxicity and mutagenicity.Experientia37, 16–17 (1981).
Lee Y.P., Takahashi T.: An improved colorimetric determination of amino acids with the use of ninhydrin.Anal. Biochem.14, 71–77 (1966).
Mashaly R.I., El-Deeb S.A.: Effect of oxygen illumination and sodium chloride on the aflatoxins produced byAspergillus, pp. 477–483 inProc. Int. Symp. Mycotoxins, Cairo (Egypt) 1983.
Miller K., Leschine S.B.: A halotolerantPlanococcus from antarctic dry valley soil.Curr. Microbiol.11, 205–210 (1984).
Morooka N., Nakano S., Itoi N., Ueno Y.: The chemical structure and the mutagenicity of emodin metabolites.Agric. Biol. Chem.54, 1247–1252 (1990).
Moubasher A.H., Abel-Hafez S.I., El-Maghraby O.M.O.: Studies on soil mycoflora of wadi. Bir-El-Ain, Eastern Dessert, Egypt.Cryptog. Mycol.6, 129–144 (1985).
Muller R., Widemann O.: Die Bestimmung des Nitrations in Wasser.Jb. V. Wasser22, 247–271 (1955).
Nabney J., Nesbitt B.F.: A spectrophotometric method for determining the aflatoxins.Analyst90, 155–160 (1965).
Naguib M.I.: Effect of ascorbic acid on respiration and carbohydrate metabolism ofCunninghamella sp.Folia Microbiol.10, 215–223 (1965).
Ostle B.:Statistics in Research. The Iowa State University Press, Ames (Iowa, USA) 1963.
Penning de Vries F.W.T.: The cost of maintenance processes in plant cells.Ann. Bot.39, 77–92 (1975).
Pitt J.I.:A Laboratory Guide to Common Penicillium Species. Commonwealth Scientific and Industrial Research Organization, Division of Food Research, Australia 1985.
Rabie C.J., Terblanche M.: Influence of temperature on the toxicity of different isolates ofAspergillus wentiiWehmer.South Afric. J. Agric. Sci.10, 263–266 (1967).
Raper K.B., Fennell D.I.:The Genus Aspergillus. Williams and Wilkins, Baltimore (USA) 1965.
Reddy M.P., Vora A.B.: Effect of salinity on protein metabolism in bajora (Pennisetum typhoides) leaves.Indian J. Plant. Physiol.28, 190–198 (1985).
Roberts R.G., Robertson J.A., Hanlin R.T.: Fungi occurring in the achenes of sunflower (Helianthus annuus).Can. J. Bot.64, 1964–1971 (1985).
Rusul G., El-Gazzar F.E., Marth E.H.: Growth of and aflatoxin production byAspergillus parasiticus in a medium containing potassium chloride or a mixture of potassium chloride and sodium chloride.J. Food. Prot.49, 880–885 (1986).
Saber S.M.: Mycotoxin production by halophilic and osmophilic fungi of different seeds in Egypt.PhD Thesis. Faculty of Science, Assiut University (Egypt) 1987.
Sassa T.: Overproduction of moniliphenone, a benzophenone biosynthetic intermediate of chloromonilicin,Monilinia fructicola and its anthraquinone precursors.Agric. Biol. Chem.55, 95–99 (1991).
Schwarz M., Gale J.: Maintenance respiration and carbon balance of plants at low levels of sodium chloride salinity.J. Exp. Bot.32, 939–942 (1981).
Shih C.N., Marth E.H.: Production of aflatoxin in a medium fortified with sodium chloride.J. Dairy Sci.55, 1415–1419 (1972).
Sinha K.K., Sinha A.K.: Effect of aflatoxin B1 on germination index and seedling growth in wheat varieties.Mycopathologia123, 165–169 (1993).
Stewart C.R., Hanson A.D.: Proline accumulation as a metabolic response to water stress, pp. 173–189 in N.C. Turner, P.J. Kramer (Eds.):Adaptation of Plants to Water and High Temperature Stress. Wiley and Sons, New York 1980.
Varley H., Gowenlock A.H., Bell M.: Lipids and lipoproteins, pp. 625–684 in H. Varley (Ed.):Practical Clinical Biochemistry. William Heinemann Medical Books, London 1980.
Vogel A.I.:Quantitative Inorganic Analysis ELBS. Longman, London 1968.
Wells J.M., Cole R.J., Kirksey J.W.: Emodin, a toxic metabolite ofAspergillus wentii isolated from weevil-damaged chestnuts.Appl. Microbiol.30, 26–28 (1975).
Zöllner N., Kirsch K.: Über die quantitative Bestimmung von Lipoiden (Mikromethods) mittels der vielen natürlichen Lipoiden (allen bekannten Plasmalipoiden) gemeinsamen Sulphophosphovanillin-Reaktion.Z. Ges. Exp. Med.135, 545–561 (1962).
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Hasan, H.A.H. Studies on toxigenic fungi in roasted foodstuff (Salted seed) and halotolerant activity of emodin-producingAspergillus wentii . Folia Microbiol 43, 383–391 (1998). https://doi.org/10.1007/BF02818578
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DOI: https://doi.org/10.1007/BF02818578