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Biochemical, morphological and cytochemical studies of enhanced autolysis ofSaccharomyces cerevisiae

2. Morphological and cytochemical studies

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Abstract

Morphological and cytochemical observation ofSaccharomyces cerevisiae undergoing of induced autolysis were done in response to various chemical inducers of autolysis (NaCl, ethanol, fresh autolyzate). Changes in the inner structure of yeast cells were monitored by transmission electron microscopy and the surface of the cell wall was observed by scanning electron microscopy during autolysis. Cytochemical characterization of autolyzed cells was performed using four synthetic substrates for determination of proteinase activities but only carboxypeptidase Y could be detected in the vacuolar membranes. The morphological studies supported the data obtained from biochemical studies and confirmed that optimized conditions of autolysis have a significant effect on the structural changes of autolyzed yeast.

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Authors and Affiliations

  1. Department of Biochemical Technology, Slovak Technical University, 812 37, Bratislava, Slovakia

    R. Kollár & E. Šturdík

  2. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague 4, Czech Republic

    J. Voříšek

Authors
  1. R. Kollár
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  2. J. Voříšek
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  3. E. Šturdík
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Kollár, R., Voříšek, J. & Šturdík, E. Biochemical, morphological and cytochemical studies of enhanced autolysis ofSaccharomyces cerevisiae . Folia Microbiol 38, 479–485 (1993). https://doi.org/10.1007/BF02814399

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  • DOI: https://doi.org/10.1007/BF02814399

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