Summary
Allerations in the localization of acid phosphatase inSaccharomyces cerevisiae during glucose repression and during autolysis have been studied. Cell morphology becomes distinctly changed after only 2 h in the presence of high glucose concentration while after 3 h of glucose repression the majority of the mitochondirial structures resemble promitochondria. Yeast cells repressed for 6 h contain almost completely degraded mitochondrial structures and numerous lipid droplets in the central vacuole and cytoplasm. Destruction of mitochondria is accompanied by the accumulation of acid phosphatase in these organelles and in the cytoplasm whereas its activity in the central vacuole is lowered, most probably because of the leakage of the enzyme into the cytoplasm.
No preferential breakdown of mitochondria is observed during autolysis. On the contrary, mitochondria are apparently the last to be degraded. Digestion of cytoplasmic regions and membranous elements occurs intravacuolarly after sequestration by protrusions of the central vacuole which are formed at the initial stages of autolysis. Acid phosphatase is not released from the central vacuole, suggesting indirectly that vacuole enzymes do not migrate into the cytoplasm during autolysis.
Similar content being viewed by others
References
Belitzer, N. V. (1972) The vacuolar system as the lysosomal apparatus of the plant cell.Doklady Akad. Nauk SSSR 203, 211–3.
Cartledge, T. G. &Lloyd, D. (1972) Subcellular fractionation of particles containing acid hydrolases fromSaccharomyces carlsbergensis.Biochem. J. 126, 755–7.
Dharmalingam, K. &Jayaraman, J. (1971) Mechanism of glucose repression of mitochondriogenesis: induction of phospholipases.Biochem. biophys. Res. Commun. 45, 1115–8.
Duntze, W., Neumann, D. &Holzer, H. (1968) Glucose induced inactivation of malate dehydrogenase in intact yeast cells.Eur. J. Biochem. 3, 326–31.
Crosman, S., Cobley, J., Hogue, P. K., Kearney, E. B. &Singer, T. P. (1973) Relation of phospholipase D activity to the decay of succinate dehydrogenase and covalently bound flavin in yeast cells undergoing glucose repression.Archs Biochem. Biophys. 158, 744–53.
Holzer, H. (1976) Catabolite inactivation in yeast.Trends Biochem. Sci. 1, (8), 178–80.
Holzer, H. (1977) Characterization and function of intracellular proteinases and proteinase inhibitors from yeast.Acta biol. med. germ. 36, 1523–4.
Kleinig, H., Steinki, Ch., Kopp, C. &Zaar, K. (1978) Oleosomes (spherosomes) fromDaucus carota suspension culture cells.Planta 140, 233–7.
Leigh, R. A. (1979) Do plant vacuoles degrade cytoplasmic components?Trends Biochem. Sci. 4, (2) N37-N38.
Luzikov, V. N., Zubatov, A. S., Rainina, E. I. &Bakeyeva, L. E. (1971) Degradation and restoration of mitochondria upon deaeration and subsequent aeration of aerobically grownSaccharomyces cerevisiae cells.Biochim. biophys. Acta 245, 321–34.
Luzikov, V. N. (1979) Degradative processes in yeast mitochondria and their relation to mitochondrial biogenesis.FEBS Lett. 53, 133–142.
Matur, A. &Berrya, D. R. (1978) The use of step enzymes as markers during meiosis and ascospore formation inSaccharomyces cerevisiae.J. Gen. Microbiol. 109, 205–13.
Mian, F. A., Kuenzi, M. T. &Halvorson, H. O. (1973) Studies on mitochondrial membrane proteins inSaccharomyces cerevisiae under different degress of glucose repression.J. Bacteriol. 115, 876–81.
Miura, T. &Yanagita, T. (1972) Cellular senescence in yeast caused by carbon-source starvation. I. Changes in activity of respiratory system and lipid peroxidation activity.J. Biochem. 72, 141–8.
Neal, W. K., Hoffman, H.-P., Price, C. A. &Avers, C. J. (1970) Derepression of mitochondria in yeast spheroplasts.Biochem. biophys. Res. Commun. 38, 414–22.
Plattner, H. &Schatz, G. (1969) Promitochondria of anaerobically grown yeast. 3. Morphology.Biochemistry 8, 339–46.
Rainina, E. I., Zubatov, A. S., Buchwalow, I. B. &Luzikov, V. N. (1979) A cytochemical study of the localization of acid phosphatase inSaccharomyces cerevisiae at different growth phases.Histochem. J. 11, 299–310.
Van Rijn, H. J. M., Linnemans, W. A. M. &Boyer, P. (1975) Localization of acid phosphatase in protoplasts fromSaccharomyces cerevisiae.J. Bacteriol. 123, 1144–9.
Saheki, T. &Holzer, H. (1975) Proteolytic activities in yeast.Biochim. biophys. Acta 384, 203–14.
Travis, D. F. &Travis, A. (1972) Ultrastructural changes in the left ventricular rat myocardial cell with age.J. Ultrastruct. Res. 39, 124–48.
Watson, K., Haslam, Y. M. &Linnane, A. W. (1970) The isolation of mitochondrial structures from anaerobically grownSaccharomyces cerevisiae.J. Cell Biol. 46, 88–98.
Yotsuyanagi, Y. (1962) Etudes sur le chondriome de la levure. I. Variation de l'ultrastructure de chondriome au course du cycle de la croissance aerobil.J. Ultrastruct. Res. 7, 121–40.
Zubatov, A. S., Rainina, E. I., Bakeyeva, L. E. &Luzikov, V. N. (1971) Degradation ofSaccharomyces cerevisiae mitochondria during deaeration of aerobically grown cells.Biokhimiya (U.S.S.R.) 36, 1156–60.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rainina, E.J., Zubatov, A.S. & Luzikov, V.N. Glucose repression and autolysis ofSaccharomyces cerevisiae cells: alterations in the cytochemical localization of acid phosphatase. Histochem J 12, 57–69 (1980). https://doi.org/10.1007/BF01066537
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01066537