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Isoelectric point determination of immobilized enzymes employing polyanionic and polycationic enzyme inhibitors as tools

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Abstract

A simple method based on the principle of Wills has been developed for the isoelectric point (pI) determination of immobilized enzymes. This approach has been applied in the case of five alkylamine immobilized enzymes, i.e., sweet potato β-amylase,B. subtilis α(-amylase, yeast invertase, bacterial dextranase andTrichoderma cellulase employing both polyanionic inhibitors like suramin, inorganic polyphosphates, dextran sulphate and polyanethol sulphonate and polycationic inhibitors like poly-L-arginine, poly-L-ornithine, and poly-L-histidine. The overall competence limitations of the method have been discussed.

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Authors and Affiliations

  1. Division of Biochemistry, Sher-E-Kashmir University of Agricultural Sciences and Technology, 190 011, Srinagar, India

    V. Ramesh

  2. Division of Biochemistry, Central Drug Research Institute, 226 001, Lucknow, India

    C. Singh & S. S. Singhal

Authors
  1. V. Ramesh
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  2. C. Singh
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  3. S. S. Singhal
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Ramesh, V., Singh, C. & Singhal, S.S. Isoelectric point determination of immobilized enzymes employing polyanionic and polycationic enzyme inhibitors as tools. Appl Biochem Biotechnol 16, 191–199 (1987). https://doi.org/10.1007/BF02798366

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