Abstract
It has been suggested that reactive oxygen metabolites and trace elements play some role in the etiology and pathogenesis of rheumatoid arthritis (RA). Superoxide dismutase (SOD) is believed to exert an important protective role against oxygen toxicity. The aim of the study was to investigate the probable changes in the levels of trace elements and SOD activity in RA. Plasma and erythrocyte copper, zinc, and magnesium levels and erythrocyte SOD activity were measured in groups of controls and RA cases. Significantly increased erythrocyte SOD activity was found in RA patients in comparison with controls(p < 0.0001). A rise in erythrocyte Zn level(p < 0.0001) and plasma Cu level(p < 0.0001) and a decrease in erythrocyte Cu level(p < 0.05) and plasma Zn level(p < 0.05) were obtained in RA patients when compared to controls. Plasma and erythrocyte Mg levels of the RA patients showed slight and statistically insignificant reductions when compared to controls(p > 0.05). In RA patients, there were positive correlations between erythrocyte SOD activity and Mg level (r = 0.4345,p < 0.01) and between erythrocyte Zn level and plasma Cu level(r = 0.4132,p < 0.01). There were negative correlations between erythrocyte SOD activity and plasma Zn level(r =-0.3605,p < 0.05) and between plasma Zn level and erythrocyte Cu level(r =-0.4578,p < 0.01) in RA patients.
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References
P. Biemond, A. J. G. Swaak, and J. F. Koster, Protective factors against oxygen free radicals and hydrogen peroxide in rheumatoid arthritis synovial fluid,Arthr. Rheum. 27, 760–765 (1984).
B. Halliwell and J. M. C. Gutteridge, Role of free radicals and catalytic metal ions in human disease: an overview,Methods Enzymol. 186, 1–85 (1990).
J. M. C. Gutteridge, Biological origin of free radicals, and mechanisms of antioxidant protection,Chem. Biol. Interact. 91, 133–140 (1994).
A. Petkau, Scientific basis for the clinical use of superoxide dismutase,Cancer Treat. Rev. 13, 17–44 (1986).
V. Honkanen, C. H. Lamberg-Allardt, and M. K. Vesterinen, Plasma zinc and copper concentrations in rheumatoid arthritis,Am. J. Clin. Nutr. 54(6), 1082–1086 (1991).
M. A. Cimmino, L. Pane, M. Cutolo, T. Valente, and S. Rovida, Zinc concentrations in rheumatoid and psoriatic arthritis,Scand. J. Rheum. 15, 403–407 (1986).
R. Milanino, A. Prigo, L. M. Bambara, M. Marrella, U. Moretti, M. Pasqualiccho, et al., Copper and zinc status in rheumatoid arthritis: studies of plasma, erythrocytes, and urine, and their relationship to disease activity markers and pharmacological treatment,Clin. Exp. Rheum. 11, 271–281 (1993).
K. Svenson, R. Hallgren, E. Johansson, and U. Lindh, Abnormal calcium, magnesium and zinc stores in peripheral blood cells from patients with inflammatory connective tissue disease,Acta Pharmacol. Toxicol. (Copenhagen) 59(Suppl.7), 386–91 (1986).
C. C. Winterbourn, R. E. Hawkins, and M. Brian, The estimation of red cell superoxide dismutase activity,J. Lab. Clin. Med. 85(2), 337–341 (1975).
Perkin-Elmer, Analysis of serum determination of copper and zinc (BC-5), inAnalytical Method for Atomic Absorption Spectrophotometry, Perkin-Elmer Corp., Norwalk, CT, p. BC-5 (1973).
F. Rosner and P. C. Gorfien, Erythrocyte and plasma zinc and magnesium levels in health and disease,J. Lab. Clin. Med. 72(2), 213–219 (1968).
A. Cennetti, M. De-Martino, E. Graziani, S. Maddali-Bongi, C. Palemo, E. Pavar, et al., Superoxide anion production by circulating polymorphonuclear leucocytes in rheumatoid arthritis,Clin. Rheum. 9(1), 51–55 (1990).
A. Stewart, Anti-inflammatory agents as inhibitors of prostaglandin synthesis in man,Med. Clin. North Am. 65(4), 713–757 (1984).
T. Westermarck, V. Honkanen, H. Mussalo-Rauhamaa, J. Lehto, P. Pelkonen, and U. R. Nordberg, Glutathione peroxidase and superoxide dismutase in juvenile rheumatoid arthritis, inElements in Health and Disease, Second International Conference on Element in Health and Disease, 6–10 February (1987), H. M. Said, A. Rahman, and L. A. D’Silva, eds., Hamdard University Press, Karachi, Pakistan, pp. 624–629 (1987).
A. Imadaya, K. Terasawa, and H. Tosa, Erythrocyte antioxidant enzymes are reduced in patients with rheumatoid arthritis,J. Rheum. 15, 1628–1631 (1988).
J. C. Banford, D. H. Brown, and R. A. Hazelton, Serum copper and erythrocyte superoxide dismutase in rheumatoid arthritis,Ann. Rheum. Dis. 41, 458–62 (1982).
P. Scudder, J. Stocks, and T. L. Dormandy, The relationship between erythrocyte superoxide dismutase activity and erythrocyte copper levels in normal subjects and in patients with rheumatoid arthritis,Clin. Chim. Acta 69, 397–403 (1976).
I. Kushner, Regulation of acute phase response by cytokines,Perspect. Biol. Med. 36(4), 611–622 (1993).
M. Marrella, U. Moretti, M. Pasqualicchio, G. P. Velo, E. Trevisani, L. M. Bambara, et al., Plasma and total blood cell copper in rheumatoid arthritis,Agents Actions 29, 120–121 (1990).
H. özgüneŞ, H. Gürer, and S. Tuncer, Correlation between plasma malondialdehyde and ceruloplasmin activity values in rheumatoid arthritis,Clin. Biochem. 28, 193–194 (1995).
C. Algre, J. Bare, and J. Obach, Zinc and rheumatic disease,Arthr. Rheum. 27(9), 1073–1074 (1984).
H. Morgenstern and F. Machtey, Serum zinc and copper levels in rheumatoid arthritis,Arthr. Rheum. 26, 933–934 (1983).
Z. Balogh, A. F. Ghobarey, G. S. Fell, D. H. Brown, J. Dunlop, and W. C. Dick, Plasma zinc and its relationship to clinical symptoms and drug treatment in rheumatoid arthritis,Ann. Rheum. Dis. 39, 329–332 (1980).
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Tuncer, S., Kamanli, A., AkÇil, E. et al. Trace element and magnesium levels and superoxide dismutase activity in rheumatoid arthritis. Biol Trace Elem Res 68, 137–142 (1999). https://doi.org/10.1007/BF02784402
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DOI: https://doi.org/10.1007/BF02784402