Summary
The tracing of ammonium nitrogen is important in hepatic diseases, especially in liver cirrhosis with hyperammonemia. In this paper, a new convenient method is described for determining stable isotope15N-labeled ammonia using the Conway apparatus and chemical ionization mass spectrometry. The minimum quantity of ammonia necessary for reliable measure of15N content was O.l μMol. The average error was 5.3%. In clinical application, 4ml of blood after oral administration of 1 g of15NH4C1 was sufficient for measurement.
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Ukida M: (in preparation)
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Ukida M: (in preparation)
Ukida M: (in preparation)
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Ukida, M., Shimada, Y. Determination of15n-ammonia by chemical ionization mass spectrometry. Gastroenterol Jpn 14, 110–113 (1979). https://doi.org/10.1007/BF02773581
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DOI: https://doi.org/10.1007/BF02773581