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Plant Molecular Biology Reporter

, Volume 21, Issue 4, pp 449–456 | Cite as

A hydroponic culture system for growingArabidopsis thaliana plantlets under sterile conditions

  • Bernhard Schlesier
  • Frédéric Bréton
  • Hans-Peter MockEmail author
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Abstract

We developed a hydroponic cultivation system for growingArabidopsis plantlets under sterile, controlled environmental conditions. The system consists of a piece of stainless-steel wire cloth (125 μm mesh size) that is fixed between 2 flat rings and held in place by 3 legs, placed in a commercially-available glass jar, and covered by the original glass lid or a sheet of sterilized cellophane. Sterilized seeds were distributed evenly across the mesh piece, the size of which allowed root growth and kept the seeds in place. After 3 weeks of cultivation, shoot and root tissues were easily harvested without mechanical damage. Proteome and metabolite analyses were performed on root and shoot tissues and demonstrated excellent reproducibility, indicating that the system is advantageous when biological variation is minimized. Induction experiments can be performed by transferring the apparatus (with plants) to a new jar containing a different nutrient solution. The apparatus is reusable and can easily be sterilized by autoclaving or dry heat. The system can be adapted to other small-seed plants by varying the mesh size.

Key words

Arabidopsis metabolic profile proteome roots sterile hydroponic culture 

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Copyright information

© International Society for Plant Molecular Biology 2003

Authors and Affiliations

  • Bernhard Schlesier
    • 1
  • Frédéric Bréton
    • 1
  • Hans-Peter Mock
    • 1
    Email author
  1. 1.Institute of Plant Genetics and Crop Plant ResearchGaterslebenGermany

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