Skip to main content
SpringerLink
Log in

Vascular effects of endothelin-1 in stage 21 chick embryos

  • Originals
  • Published:
Heart and Vessels Aims and scope Submit manuscript

Summary

Endothelin-1 is a very potent vasoconstrictor, but its function has not yet been investigated in the early stage of cardiovascular development. The purpose of the present study was to clarify whether endothelin-1 exerts a hemodynamic effect in stage 21 chick embryos. We measured vitelline artery blood pressure with a servo-null micropressure system and blood flow velocity at the dorsal aorta with a 20 MHz pulsed Doppler velocity meter. The vitelline vessels were directly measured with a microscope video system. While monitoring these parameters, endothelin-1 was infused into a vein by a microinjector and data were collected. Endothelin-1 increased the blood pressure and heart rate, but decreased the dorsal aortic blood flow. Only the vitelline veins with a diameter of between 100 and 200µm constricted after endothelin infusion, but smaller or larger veins and the arteries did not show any significant change in size, although the resistant arteries could not be measured by this method. In conclusion, endothelin-1 has apparent constrictive effects in the selected vessel in the early stages of cardiovascular development when the endocrine and autonomic nervous systems have not yet developed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wagman AJ, Hu N, Clark EB (1990) Effect of changes in circulating blood volume on cardiac output and arterial and ventricular blood pressure in the stage 18, 24, and 29 chick embryo. Circ Res 67:187–192

    PubMed  CAS  Google Scholar 

  2. Wispe J, Hu N, Clark EB (1983) Effect of environmental hypothermia on dorsal aortic blood flow in the chick embryo, stages 18 to 24. Pediatr Res 17:945–948

    Article  PubMed  CAS  Google Scholar 

  3. Nakazawa M, Miyagawa S, Takao A, Clark EB, Hu N (1986) Hemodynamic effect of environmental hyperthermia in stage 18, 21, and 24 chick embryos. Pediatr Res 20:1213–1215

    Article  PubMed  CAS  Google Scholar 

  4. Nakazawa M, Kajio F, Ikeda K, Takao A (1990) Effect of atrial natriuretic peptide on hemodynamics of the stage 21 chick embryo. Pediatr Res 27:557–560

    Article  PubMed  CAS  Google Scholar 

  5. Clark EB, Hu N, Dooley JB (1984) The effect of isoproterenol on cardiovascular function in the stage 24 chick embryo. Teratology 31:41–47

    Article  Google Scholar 

  6. Tomita H, Nakazawa M, Nishibatake M, Ikeda K, Takao A (1989) Effect of isoproterenol on heart volume in chick embryos (in Japanese). Acta Cardiol Paed Jpn 5:286–290

    Google Scholar 

  7. Hoffman LE Jr, VanMierop LHS (1971) Effect of epinephrine on heart rate and arterial blood pressure of the developing chick embryo. Pediatr Res 5:472–477

    Article  CAS  Google Scholar 

  8. Girard H (1973) Adrenergic sensitivity of circulation in the chick embryo. Am J Physiol 224:461–469

    PubMed  CAS  Google Scholar 

  9. Petery LB Jr, VanMierop LHS (1977) Evidence for the presence of adrenergic receptors in 3-day-old chick embryo. Am J Physiol 232:H250-H254

    PubMed  CAS  Google Scholar 

  10. Kurihara Y, Kurihara H, Suzuki H, Kodama T, Maemura K, Nagai R, et al (1994) Elevated blood pressure and craniofacial abnormalities in mice deficient in endothelin-1. Nature 368:703–710

    Article  PubMed  CAS  Google Scholar 

  11. Kurihara Y, Kurihara H, Oda H, Maemura K, Nagai R, Ishikawa T, et al (1995) Aortic arch malformations and ventricular septal defect in mice deficient in endothelin-1. J Clin Invest 96:293–300

    Article  PubMed  CAS  Google Scholar 

  12. Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92

    Article  Google Scholar 

  13. Clark EB, Hu N (1982) Developmental hemodynamic changes in the chick embryos from stage 18 to 27. Circ Res 51:810–815

    PubMed  CAS  Google Scholar 

  14. Mancini GB, Simon SB, McGillem MJ, LeFree MT, Friedman HZ, Vogel RA (1987) Automated quantitative coronary arteriography: morphologic and physiologic validation in vivo of a rapid digital angiographic method. Circulation 75:452–460

    PubMed  CAS  Google Scholar 

  15. Nakazawa M, Miyagawa S, Nishibatake M, Ikeda K, Takao A (1988) Hemodynamic characteristics in neural crest cell-excised chick embryo. Heart Vessels 4:136–140

    Article  PubMed  CAS  Google Scholar 

  16. Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, et al (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415

    Article  PubMed  CAS  Google Scholar 

  17. Clark EB (1987) Mechanisms in the pathogenesis of congenital cardiac malformations. In: Pierpont MEM, Moller JH (eds) Genetics of cardiovascular disease. Martinus Nijhoff, Boston, pp 3–12

    Google Scholar 

  18. Dashwood MR, Timm M, Kaski JC (1995) Regional variations in ETA/ETB binding sites in human coronary vasculature. J Cardiovasc Pharmacol 26 [Suppl 3]:S351-S354

    PubMed  CAS  Google Scholar 

  19. Hergenroder S, Munter K, Kirchengast M (1995) Effect of endothelin-1 and BQ 123 on arterioles and venules of hypertensive rats. J Cardiovasc Pharmacol 26 [Suppl 3]:S226-S229

    PubMed  Google Scholar 

  20. Haynes WG, Strachan FE. Webb DJ (1995) Endothelin ETA and ETB receptors cause vasoconstriction of human resistance and capacitance vessels in vivo. Circulation 92:357–363

    PubMed  CAS  Google Scholar 

  21. Wang Y, Coceani F (1992) Isolated pulmonary resistance vessels from fetal lambs: contractile behavior and responses to indomethacin and endothelin-1. Circ Res 72:320–330

    Google Scholar 

  22. Perreault T, Baribeau J (1995) Characterization of endothelin receptors in newborn piglet lung. Am J Physiol 268(4 Pt 1):L607-L614

    PubMed  CAS  Google Scholar 

  23. Zellers TM, McCormick J, Wu Y (1994) Interaction among ET-1, endothelium-derived nitric oxide, and prostacyclin in pulmonary arteries and veins. Am J Physiol 267:H139-H147

    PubMed  CAS  Google Scholar 

  24. Takai K, Ito H, Sahashi T, Wada H, Segawa T, Hirakawa S (1992) Differing actions of endothelin-1 on canine systemic resistance and capacitance vessels. Jpn Circ J 56:847–854

    PubMed  CAS  Google Scholar 

  25. Concas V, Laurent S, Brisac AM, Perret C, Safer M (1989) Endothelin has potent direct inotropic and chronotropic effects in cultured heart cells. J Hypertens 7 [Suppl]:S96-S97

    CAS  Google Scholar 

  26. Ishikawa T, Li LM, Shinmi O, Kimura S, Yanagisawa M, Goto K, et al (1991) Characteristics of binding of endothelin-1 and endothelin-3 to rat hearts. Developmental changes in mechanical responses and receptor subtypes. Circ Res 69:918–926

    PubMed  CAS  Google Scholar 

  27. Siegfried MR, Aoki N, Mulloy D, Lefer AM (1990) Direct positive inotropic and vasoconstrictor effects of endothelin. Heart Vessels 5:146–151

    Article  PubMed  CAS  Google Scholar 

  28. Beyer ME, Slesak G, Hoffmeister HM (1995) In vivo hemodynamic and inotropic effects of the endothelin B agonist IRL 1620. J Cardiovasc Pharmacol 26 [Suppl 3]:S190-S192

    PubMed  CAS  Google Scholar 

  29. Pappano AJ (1977) Ontogenetic development of autonomic neuroeffector transmission and transmitter reactivity in embryonic and fetal heart. Pharmacol Rev 29:3–33

    PubMed  CAS  Google Scholar 

  30. Yoshigi M, Hu N, Keller BB (1996) Dorsal aortic impedance in stage 24 chick embryo following acute changes in circulating blood volume. Am J Physiol 270 (Heart Circ Physiol 39):H1597-H1606

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatric Cardiology, The Heart Institute of Japan, Tokyo Women’s Medical College, 8-1 Kawada-cho, Shinjuku-ku, 162-8666, Tokyo, Japan

    Fusae Kajio & Makoto Nakazawa

Authors
  1. Fusae Kajio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Makoto Nakazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kajio, F., Nakazawa, M. Vascular effects of endothelin-1 in stage 21 chick embryos. Heart Vessels 12, 300–305 (1997). https://doi.org/10.1007/BF02766807

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02766807

Key words

Search

Navigation