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A solid phase radioimmunoassay for detection of early pregnancy in the South Indian bonnet monkeyMacaca radiata

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Abstract

Using an antibody raised in the rabbit to ovine leutenizing hormone β subunit coupled to activated cellulose, a solid phase radioimmunoassay to detect early pregnancy in the South Indian bonnet monkey has been developed. Non-specific inhibition due to serum was eliminated by inclusion of new born calf serum in the assay tubes. The assay is simple, needs only one centrifugation and can be completed in 6 h at room temperature with no false positive results.

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Abbreviations

CG:

Chorionic gonadotropin

oLH:

ovine leutinizing hormone

hCG:

human CG

DEAE:

diethylaminoethyl

BSA:

bovine serum albumin

RIA:

radioimmunoassay

NCS:

new born calf serum

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Authors and Affiliations

  1. Department of Biochemistry, Center for Advanced Research in Reproductive Biology (ICMR), Indian Institute of Science, 560 012, Bangalore, India

    R. Chakrabarti, G. S. Murthy & A. Jagannadha Rao

Authors
  1. R. Chakrabarti
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  2. G. S. Murthy
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  3. A. Jagannadha Rao
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Chakrabarti, R., Murthy, G.S. & Rao, A.J. A solid phase radioimmunoassay for detection of early pregnancy in the South Indian bonnet monkeyMacaca radiata . J Biosci 7, 145–152 (1985). https://doi.org/10.1007/BF02703583

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  • DOI: https://doi.org/10.1007/BF02703583

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