Abstract
Human erythrocyte specific lectin was isolated from the seeds ofErythrina variegata Linn. var.orientalis Linn. Merrill. The lectin preferentially agglutinated erythrocytes in the sequence of O>B>A = AB. The lectin was purified 19-fold by affinity chromatography on acid treated sepharose 4B with an yield of 81%. The purified lectin was found homogeneous on polyacrylamide gel electrophoresis. The erythroagglutination reaction was inhibited by N-acetyl-D-galactosamine, D-galactose and lactose at very low concentration. The haemagglutination by the purified lectin was not inhibited by different hexose and pentose sugars even at high concentration. The purified lectin was a glycoprotein and agglutinated leucocytes at 3 μg protein concentration. The lectin induced transformation of peripheral blood lymphocytes in cultures.
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Abbreviations
- PBS:
-
Phosphate buffered saline
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Datta, T.K., Basu, P.S. Human erythrocyte specific lectin from the seeds of Indian coral tree,Erythrina variegata Linn, var.orientali Linn, Merrill. J Biosci 5 (Suppl 1), 25–30 (1983). https://doi.org/10.1007/BF02702971
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DOI: https://doi.org/10.1007/BF02702971