Skip to main content
SpringerLink
Log in

Optimization by isochronal analysis I. Changes in mobile phase composition and velocity

  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

The theory of isochronal analysis (time normalization) has been extended to reversed-phase liquid chromatography. Of the possible manipulation of experimental parameters, the present paper develops a framework for optimizing the resolution by the simultaneous change of mobile phase velocity and composition. For that purpose the resolution equation was written in terms of these two parameters and under the constraint of constant analysis time. This treatment allows plotting the resolution versus the mobile phase composition and velocity. The surfaces thus obtained show the changes that the analyst must perform in order to optimize the resolution. The treatment deals with three possible α dependencies on the amount of organic modifier: constant, increasing and decreasing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. Guiochon, in “High Performance Liquid Chromatography, Advances and Perspectives”,C. Horvath, ed., Academic Press: New York, 1980.

    Google Scholar 

  2. I. Halasz, G. Gortilz, Angew. Chem. Int., Ed., Engl.21, 50 (1982).

    Article  Google Scholar 

  3. B. Sachok, R. C. Kong, S. N. Deming, J. Chromatogr.199, 317 (1980).

    Article  CAS  Google Scholar 

  4. B. Sachok, R. C. Kong, S. N. Deming, J. Chromatogr.199, 307 (1980).

    Article  Google Scholar 

  5. L. R. Snyder, J. J. Kirkland, “An Introduction to Modern Liquid Chromatography”, 2nd ed., Wiley: N.Y. 1979.

    Google Scholar 

  6. L. Rohrschneider, Anal. Chem.45, 1241 (1973).

    Article  CAS  Google Scholar 

  7. L.R. Snyder, J. Chromatogr. Sci.16, 223 (1978).

    CAS  Google Scholar 

  8. P. J. Schoenmakers, H. A. H. Billiet, L. J. de Galan, J. Chromatogr.185, 179 (1979).

    Article  CAS  Google Scholar 

  9. N. Tanaka, N. Goodell, B. L. Karger, J. Chromatogr.158, 233 (1978).

    Article  CAS  Google Scholar 

  10. S. R. Bakalyar, R. Mcilwrick, E. Roggendorf, J. Chromatogr.142, 353 (1977).

    Article  CAS  Google Scholar 

  11. J. L. Glajch, J. J. Kirkland, K. M. Squire, J. M. Minor, J. Chromatogr.199, 57 (1980).

    Article  CAS  Google Scholar 

  12. P. E. Antle, Chromatographia15, 277 (1982).

    Article  CAS  Google Scholar 

  13. L. R. Snyder, J. L. Glajch, J. J. Kirkland, J. Chromatogr.218, 299 (1981).

    Article  CAS  Google Scholar 

  14. J. L. Glajch, J. J. Kirkland, L. R. Snyder, J. Chromatogr.238, 269 (1982).

    Article  CAS  Google Scholar 

  15. J. L. Glajch, J. J. Kirkland, Anal. Chem.55, 319A (1983).

  16. H. J. Issaq, J. Liq. Chromatogr.4, 1917–31 (1981).

    Article  CAS  Google Scholar 

  17. A. P. Goldberg, Anal. Chem.54, 342 (1982).

    Article  CAS  Google Scholar 

  18. B. L. Karger, W. D. Cooke, Anal. Chem.36, 985 (1964).

    Article  CAS  Google Scholar 

  19. B. L. Karger, W. D. Cooke, Anal. Chem.36, 991 (1964).

    Article  CAS  Google Scholar 

  20. E. Grushka, W. D. Cooke, J. Chromatogr. Sci.9, 310 (1971).

    CAS  Google Scholar 

  21. E. Grushka, Anal. Chem.43, 766 (1971).

    Article  CAS  Google Scholar 

  22. E. Grushka, M. Yepes-Baraya, W. D. Cooke, J. Chromatogr. Sci.9, 653 (1971).

    CAS  Google Scholar 

  23. G. Guiochon, Anal. Chem.38, 1020 (1966).

    Article  CAS  Google Scholar 

  24. G. Guiochon, in “Column Chromatography”,E. Kovats, Ed., Sauerlander Harav, Switzerland, 250, 1970.

    Google Scholar 

  25. E. Grushka, G. Guiochon, J. Chromatogr. Sci.10, 649 (1972).

    CAS  Google Scholar 

  26. E. Grushka, J. Chromatogr. Sci.10, 616 (1972).

    CAS  Google Scholar 

  27. B. L. Karger, J. R. Gant, A. Hartkopf, P. H. Weiner, J. Chromatogr.128, 65 (1976).

    Article  CAS  Google Scholar 

  28. J. R. Gant, J. W. Dolan, L. R. Snyder, J. Chromatogr.185, 153 (1979).

    Article  CAS  Google Scholar 

  29. J. H. Knox, J. Chromatogr. Sci.15, 352 (1977).

    CAS  Google Scholar 

  30. L. R. Perkins, G. J. Geankoplis, Chem. Eng. Sci.24, 1035 (1969).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Inorganic Chemistry and Analytical Chemistry, The Hebrew University, Jerusalem, Israel

    I. Atamna & E. Grushka

  2. Laboratoire de Chemie Analytique Physique, Ecole Polytechnique, 91128, Palaiseau Cedex, France

    H. Colin & G. Guiochon

Authors
  1. I. Atamna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Grushka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Colin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Guiochon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Atamna, I., Grushka, E., Colin, H. et al. Optimization by isochronal analysis I. Changes in mobile phase composition and velocity. Chromatographia 19, 48–54 (1984). https://doi.org/10.1007/BF02687718

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02687718

Key Words

Search

Navigation