Abstract
An efficient and convenient method is presented for the isolation of nuclear DNA from leaves of wildVitis species that have been dried with silica gel. The nuclear DNA obtained with this method is suitable for both PCR amplification and digestion with restriction endonucleases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chase, M.W. and H.H. Hills. 1991. Silica gel: An ideal material for field preservation of leaf samples for DNA studies. Taxon 40:215–220.
Collins, G.G. and R.H. Symons. 1992. Extraction of nuclear DNA from grape vine leaves by a modified procedure. Plant Mol. Biol. Reptr. 10(3):233–235.
Couch, J.A. and P.J. Fritz. 1990. Isolation of DNA from plants high in polyphenolics. Plant Mol. Biol. Reptr. 8:8–12.
Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19:11–15.
Fang, G., S. Hammar and R. Rebecca. 1992. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Bio Techniques 13:52–56.
John, M.E. 1992. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nuc. Acids Res. 20:2381.
Kopperud, C. and J.W. Einset. 1995. DNA isolation from begonia leaves. Plant Mol. Biol. Reptr. 13:129–130.
Li, Q.-B., Q.-Y. Cai and C.L. Guy. 1994. A DNA extraction method for RAPD analysis from plants rich in soluble polysaccharides. Plant Mol. Biol.Reptr. 12:215–220.
Lodhi, M.A., G.-N. Ye, N.F. Weeden, and B.I. Reisch. 1994. A simple and efficient method for DNA extraction from grapevine cultivars andVitis species. Plant Mol. Biol. Reptr. 12:6–13.
Rether, B., G. Delmas and A. Laouedj. 1993. Isolation of polysaccharide-free DNA from plant. Plant Mol. Biol. Reptr. 11:333–337.
Rogers, S.U., and A.J. Bendich. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5:69–76.
Rogers, S.O. and A.J. Bendich. 1988. Extraction of DNA from plant tissues. Plant Mol. Biol. Manual A6:1–10.
Thomas, M.R., S. Matsumoto, P. Cain, and N.S. Scott. 1993. Repetitive DNA of grapevine: Classes present and sequences suitable for cultivar identification. Theor. Appl. Genet. 86:173–180.
Thomson, D. and R. Henry 1993. Use of DNA from dry leaves for PCR and RAPD analysis. Plant Mol. Biol. Reptr. 11: 202–206.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, XD., Wang, ZP. & Zou, YP. An improved procedure for the isolation of nuclear DNA from leaves of wild grapevine dried with silica gel. Plant Mol Biol Rep 14, 369–373 (1996). https://doi.org/10.1007/BF02673369
Issue Date:
DOI: https://doi.org/10.1007/BF02673369