Abstract
Flour samples were prepared from intact and degermed kernels of Hard Red Spring, Soft White Spring, and Amber Durum wheats. The “free” (hexane soluble) and “bound” (hexane resistant, water-saturatedn-butanol extractable) lipids were extracted from the six flours and separated quantitatively by silicic acid column chromatography. Thin-layer chromatography (TLC) was used to monitor the column and to resolve the lipid classes into components. Gas-liquid chromatography (GLC) was used to obtain the fatty acid composition of the triglyceride, sterol ester, and phospholipid fractions, and also to determine the nature of the sterol components of the unesterified sterol and sterol ester classes.
Similar patterns of lipid classes were shown by all three varieties; the differences were in the degree of dominance. In fatty acid composition some varietal differences were found but the greatest difference was between lipid classes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fisher, N. Recent Advances in Food Science, Vol. I, Ed. J. Hawthorn and J. M. Leitch, Butterworth's, London 1962, p. 226–245.
Cookson, M. A., and J. B. M. Coppock, J. Sci. Food Agri.7, 72 (1956).
Meredith, P., J. Sci. Food Agri.11, 320–329 (1960).
Wren, J. J., and S. C. Elliston, Chem. Ind. 80–81 (1961).
Coulson, O. B., and E. A. Somerville, Biochem J.80, 45 (1961).
Houston, D. F., Cereal Sci.6, 288, 290, 291, 300 (1961).
Stevens, D. J., Cereal Chem.36, 452–461 (1959).
Carter, H. E., R. H. McCluer, and E. D. Slifer, J Amer. Chem. Soc.78, 3735 (1956).
Carter, H. E., W. D. Celmer, D. S. Galanos, R. N. Gigg, W. E. M. Lands, J. H. Law, K. L. Mueller, T. Nakayama, H. H. Tomizawa, and E. Weber, JAOCS35, 335 (1958).
Carter, H. E., K. Ohno, S. Nojima, C. L. Tipton, and N. Z. Stanacev, J. Lipid Res.2, 215–222 (1961).
Mason, H. L., and A. E. Johnston. Cereal Chem.35, 435 (1958).
Sims, R. P. A., and J. C. Mes, in preparation.
Sahasrabudha, M. R., and D. G. Chapman. JAOCS38, 88–92 (1961).
Hirsch, J., and E. H. Ahren, Jr., J. Biol. Chem.233, 311–320 (1958).
Choudhury, R. B. R., and L. K. Arnold. JAOCS37, 87–88 (1960).
Stahl, E., Pharmazie11, 633 (1956).
Mangold, H. K., and D. C. Malins, JAOCS37, 383–385 (1960).
Marinetti, G. V., J. Erbland and J. Kochen. Fed. Proc.16, 837–844 (1957).
Wagner, R., L. Horhammer, and P. Wolf, Biochem. Z.334, 175–184 (1961).
Lepage, M., J. Chromatog, in press.
Sims, R. P. A., and J. A. G. Larose, JAOCS39, 232 (1962).
Mangold, H. K., J. L. Gellerman, and H. Schlenk, Fed. Prod.17, 268 (1958).
Rouser, C., G. V. Marinetti, R. F. Whitter, J. F. Berry, and E. Stotz, J. Biol. Chem.233, 485–497 (1956).
Bergoff, H. M., E. Roberts, and C. C. Delwiche, J. Biol. Chem.205, 565 (1953).
Bandurski, R. S., and B. Axelrod, J. Biol. Chem.193, 405 (1951).
Stoffel, W., F. Chu, and E. H. Ahrens, Jr., Anal. Chem.31, 307 (1959).
Sims, R. P. A., and J. C. Mes, in preparation.
Research Specialties Co., Chromatofacts, Sept.–Oct., 1962.
Langer, S. H., R. A. Friedel, I. Wender, and A. G. Sharkey, Jr., Anal. Chem.30, 1353 (1958).
Langer, S. H., S. Connell, and I. Wender, J. Org. Chem.,23, 50–53 (1958).
Author information
Authors and Affiliations
Additional information
Contribution No. 5 of the Food Research Institute. Presented at the AOCS Meeting, Toronto, 1962.
About this article
Cite this article
McKillican, M.E., Sims, R.P.A. The endosperm lipids of three Canadian wheats. J Am Oil Chem Soc 41, 340–344 (1964). https://doi.org/10.1007/BF02654809
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02654809