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Biochemistry of the sphingolipides. X. Phytoglycolipide, a complex phytosphingosine-containing lipide from plant seeds

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Journal of the American Oil Chemists Society

Summary

A new complex lipide, phytoglycolipide, has been isolated from soybea,, corn, flaxseed, peanut, sunflowerseed, cottonseed, and wheat phosphatides. This material is obtained by a mild alkaline saponification procedure which destroys glycerol-containing lipides. The new lipide constitutes about 5% of the crude phosphatides and is obtained as a white amorphous powder of identical composition, optical activity, and solubility properties from the various sources. Phytoglycolipide gives on hydrolysis phytosphingosine (and, in the case of soybean only, an unsaturated derivative of phytosphingosine) fatty acids, inositol, glucosamine, a hexuronic acid, galactose, arabinose, mannose, and phosphate.

Phytoglycolipide is the first sphingolipide of plant origin to be described and is unique among complex sphingolipides in that it has the structural features of a glycolipide and of a phosphatide.

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Author information

Author notes

  1. Walter D. Celmer

    Present address: Chas. Pfizer and Sons, Brooklyn, N.Y.

  2. William E. M. Lands

    Present address: Department of Biological Chemistry, University of Michigan, Ann Arbor, Mich

  3. John H. Law

    Present address: Converse Memorial Laboratory, Harvard University, Cambridge, Mass

  4. H. H. Tomizawa

    Present address: School of Medicine, University of Washington, Seattle, Wash

Authors and Affiliations

  1. Division of Biochemistry, Noyes Laboratory of Chemistry, University of Illinois, Urbana, Illinois

    Herbert E. Carter, Walter D. Celmer, Dimitris S. Galanos, R. H. Gigg, William E. M. Lands, John H. Law, Katherine L. Mueller, Teishi Nakayama, H. H. Tomizawa & Evelyn Weber

Authors
  1. Herbert E. Carter
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  2. Walter D. Celmer
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  3. Dimitris S. Galanos
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  4. R. H. Gigg
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  5. William E. M. Lands
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  6. John H. Law
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  7. Katherine L. Mueller
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  8. Teishi Nakayama
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  9. H. H. Tomizawa
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  10. Evelyn Weber
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Additional information

The soybean phosphatide work was done under contract with the U.S. Department of Agriculture and was authorized by the Research and John C. Cowan of the Northern Utilization Research and Development Division of the Agricultural Research Service, Peoria, Ill. The corn phosphatide work was supported in part by research grants (B574-C2, 3, 4) from the National Institutes of Health, United States Public Health Service. Part of the material in this paper was taken from the theses submitted by Walter D. Celmer, William E. M. Lands, John H. Law, and H. H. Tomizawa to the Graduate College of the University of Illinois in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry.

A preliminary report of this work has been published (4). For number IX in this series see reference (2)

Postdoctorate Rosearch Associate, Department of Chemistry

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Carter, H.E., Celmer, W.D., Galanos, D.S. et al. Biochemistry of the sphingolipides. X. Phytoglycolipide, a complex phytosphingosine-containing lipide from plant seeds. J Am Oil Chem Soc 35, 335–343 (1958). https://doi.org/10.1007/BF02640547

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  • DOI: https://doi.org/10.1007/BF02640547

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