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Indirect somatic embryogenesis and plant recovery from cotton (Gossypium hirsutum L.)

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Summary

We describe a tissue culture procedure for somatic embryogenesis and plantlet regeneration in cotton (Gossypium hirsutum L. cv. Coker 312). Callused explants or individual globular embryos were transferred to basal media to induce somatic embryogenesis. To determine characteristic early indicators of successful germination and conversion, we identified six types of embryos that developed on basal media. Two of the six embryo types, designated as tulip-shaped and trumpet-shaped, could undergo conversion in preliminary tests, whereas the others had little or no developmental potential. Several media treatments designed to enhance the maturation of globular somatic embryos failed to increase the fraction of embryos which matured to form recoverable types. In efforts to improve plantlet recovery, tulip-shaped embryos were used in limited trials to contrast the effects of chemical and physical desiccation treatments on germination and conversion. The selective use of tulip-shaped somatic embryos, coupled with partial desiccation, seems to have augmented plant recovery. Growth habit, flowering, seed set, and lint production of most of the regenerated plants were comparable to seed-derived plants grown under the same conditions.

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Author notes

  1. Kui Shin Voo

    Present address: Genetics Department, North Carolina State University, Raleigh, NC

  2. Clayton L. Rugh

    Present address: Botany Department, University of Georgia, Athens, GA

Authors and Affiliations

  1. Agronomy Department, The Ohio State University, 43210, Columbus, Ohio

    Kui Shin Voo & Joe C. Kamalay

  2. Botany Department, The Ohio State University, 43210, Columbus, Ohio

    Clayton L. Rugh & Joe C. Kamalay

  3. Molecular Genetics Department, The Ohio State University, 43210, Columbus, Ohio

    Joe C. Kamalay

Authors
  1. Kui Shin Voo
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  2. Clayton L. Rugh
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  3. Joe C. Kamalay
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Additional information

Partial research support was provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University.

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Voo, K.S., Rugh, C.L. & Kamalay, J.C. Indirect somatic embryogenesis and plant recovery from cotton (Gossypium hirsutum L.). In Vitro Cell Dev Biol - Plant 27, 117–124 (1991). https://doi.org/10.1007/BF02632194

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  • DOI: https://doi.org/10.1007/BF02632194

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