Summary
This note describes a simple experimental system for incubating plant cell and tissue cultures under conditions in which the gaseous environment within the culture flasks is continuously flushed out by air or gas mixtures of known composition. Two of the major problems in maintaining such cultures, namely, contamination and desccation of the agar-solidified medium, can be overcome by using this setup. Furthermore, with modifications, it can be adapted for commercial micropropagation projects.
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This research was supported by Natural Sciences and Engineering Research Council of Canada grant A-6467 to T. A. Thorpe. The authors thank R. W. Joy IV for drawing Fig. 1B.
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Kumar, P.P., Thorpe, T.A. A setup for incubating plant cultures under continuous flow of gases. In Vitro Cell Dev Biol - Plant 27, 43–44 (1991). https://doi.org/10.1007/BF02632061
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DOI: https://doi.org/10.1007/BF02632061