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Basic conditions for the drug selection and transient gene expression in the cultured cell line ofBombyx mori

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Summary

We established basic conditions for transient gene expression and selection of antibiotics in the cultured cell line of silkworm,Bombyx mori, by use of the promoter of the heat shock protein (hsp70) gene ofDrosophila melanogaster. The control promoter (hsp70) promoted the expression of chloramphenicol acetyltransferase (CAT) gene ligated at the downstream, dependent on the orientation of the promoter in the silkworm cell. The cell line is able to be supplied for the promoter assay of the silkworm genes. The concentration for the drug selection was determined as 0.75 mg/ml on neomycin analog, G418 (geneticin).

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Authors and Affiliations

  1. Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, 113, Tokyo, Japan

    Kazuhiro Okano & Masahiko Kobayashi

  2. Department of Technology, National Institute of Health, 2-10-35, Kamiosaki, Shinagawa-ku, 141, Tokyo, Japan

    Naoko Miyajima, Naoko Takada & Hideaki Maekawa

Authors
  1. Kazuhiro Okano
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  2. Naoko Miyajima
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  3. Naoko Takada
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  4. Masahiko Kobayashi
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  5. Hideaki Maekawa
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Okano, K., Miyajima, N., Takada, N. et al. Basic conditions for the drug selection and transient gene expression in the cultured cell line ofBombyx mori . In Vitro Cell Dev Biol - Animal 28, 779–781 (1992). https://doi.org/10.1007/BF02631067

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  • DOI: https://doi.org/10.1007/BF02631067

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