Skip to main content
SpringerLink
Log in

Investigations on isolated islets in vitro. IX. Influence of food deprivation and glucose refeeding in vitro on insulin secretion

  • Published:
Acta diabetologia latina Aims and scope Submit manuscript

Summary

Starvation resulted in a progressive decrease of insulin secretion in response to 15 mM glucose, but it neither inhibited insulin release completely nor changed the shape of the glucose response curve. Long-term fasting caused the glucose threshold to rise to 30 mM glucose during an incubation time of 15 min, whereas fed animals were characterized by a sigmoidal insulin response and a threshold level of 5 mM glucose during short-term incubation, too. Prolongation of incubation time (60 or 120 min) or pretreatment of isletsin vitro with 20 mM glucose for 60 min caused progressive lowering of the glucose threshold, indicating that glucoseper se influenced the effective stimulating concentration. The effect of starvation is not specific for glucose, since hormone secretion in response to glibenclamide, nicotinic acid and theophylline was also reduced, whereas theophylline can partly counteract the diminished release. Although the content of insulin as well as glucagon in the islets was reduced by food deprivation it is assumed that the islet hormone content is not responsible for the rise of the glucose threshold caused by starvation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ammon H. P. T., Steinke J.: 6-Aminonicotinamide (6-AN) as a Diabetogenic Agent. In Vitro and in Vivo Studies in the Rat — Diabetes21, 143, 1972.

    PubMed  CAS  Google Scholar 

  2. Andersson A., Hellerström C.: Metabolic Characteristics of Isolated Pancreatic Islets in Tissue Culture — Diabetes21 (Suppl. 2), 546, 1972.

    PubMed  CAS  Google Scholar 

  3. Bosboom R. S., Zweens J., Bouman P. R.: Effects of Feeding and Fasting on the Insulin Secretory Response to Glucose and Sulfonylureas in Intact Rats and Isolated Perfused Rat Pancreas — Diabetologia9, 243, 1973.

    Article  PubMed  CAS  Google Scholar 

  4. Buchanan K. D., Vance J. E., Williams R. H.: Effect of Starvation on Insulin and Glucagon Release from Isolated Islets of Langerhans of the Rat — Metabolism18, 155, 1969.

    Article  PubMed  CAS  Google Scholar 

  5. Cerasi E., Luft R.: Insulin Response to Glucose Infusion in Diabetic and Non-Diabetic Monozygotic Twin Pairs. Genetic Control of Insulin Response? — Acta endocr. (Kbh.)55, 330, 1967.

    CAS  Google Scholar 

  6. Cerasi E., Luft R.: The Effect of an Adenosine-3′,5′-Monophosphate Diesterase Inhibitor (Aminophylline) on the Insulin Response to Glucose Infusion in Prediabetic and Diabetic Subjects — Hormone metab. Res.1, 162, 1969.

    Article  CAS  Google Scholar 

  7. Cerasi E., Luft R.: The Prediabetic State, its Nature and Consequences: a Look toward the Future — Diabetes21 (Suppl. 2), 685, 1972.

    PubMed  CAS  Google Scholar 

  8. Deery D. J., Taylor K. W.: Effects of Azaserine and Nicotinamide on Insulin Release and Nicotinamide-Adenine Dinucleotide Metabolism in Isolated Rat Islets of Langerhans — Biochem. J.134, 557, 1973.

    PubMed  CAS  Google Scholar 

  9. Fiedler H., Hahn H. J., Ziegler B., Ziegler M., Jutzi E., Michael R.: Do Different Mechanisms of Insulin Release Depend on the Feeding States? — Diabetologia7, 479, 1971.

    Google Scholar 

  10. Fischer U., Hommel H., Köhler E.: Some Metabolic Data on Blood and on Isolated Rat Tissues after Starvation Periods of Different Length — Acta biol. med. germ.27, 499, 1971.

    PubMed  CAS  Google Scholar 

  11. Gottschling D., Ziegler M., Wilke W., Michael R.: Radioimmunoassay von Plasmainsulin — methodenkritische Untersuchungen — Radiobiol. Radiother. (Berl.)15, 91, 1974.

    CAS  Google Scholar 

  12. Grey N. J., Goldring S., Kipnis D. M.: The Effect of Fasting Diet and Actinomycin D on Insulin Secretion in the Rat — J. clin. Invest.49, 881, 1970.

    Article  PubMed  CAS  Google Scholar 

  13. Hahn H.-J., Hellman B., Lernmark Å., Sehlin J., Täljedal J.-B.: The Pancreatic β-Cell Recognition of Insulin Secretagogues Influence of Neuraminidase Treatment on the Release and the Islet Content of Insulin Sialic Acid and Adenosine 3′,5′-Cyclic Monophosphate — J. biol. Chem.249, 5275, 1974.

    PubMed  CAS  Google Scholar 

  14. Hahn H.-J., Hellman B., Lernmark Å., Täljedal J.-B.: Veränderung der Insulinsekretion isolierter Langerhans’scher Inseln durch Behandlung mit Neuraminidase — Acta biol. med. germ.32, 375, 1974.

    PubMed  CAS  Google Scholar 

  15. Hahn H.-J., Lippmann H. G., Knospe S., Michael R.: Verhalten der Langerhans’schen Inseln des Pankreas in vitro. III. Insulinsekretionsrate in Abhängigkeit von der Überlebensrate und von der Glukosekonzentration im Medium — Acta biol. med. germ.25, 573, 1970.

    PubMed  CAS  Google Scholar 

  16. Hahn H.-J., Michael R.: Influence of Nicotinic Acid on Insulin Release in Vitro — Acta diabet. lat.9, 87, 1972.

    Article  CAS  Google Scholar 

  17. Hahn H.-J., Ziegler B., Butter R., Ziegler M., Fiedler H.: Insulinsekretion isolierter Langerhans’scher Inseln der Ratte bei unterschiedlicher metabolischer Stimulation in vitro — In:Bibergell H., Fiedler H., Poser U. (Eds.): Insulin Biosynthese und Sekretion. VII. Karlsburger Symposium über Diabetesfragen. Zentralinstitut für Diabetes « Gerhardt Katsch », Karlsburg, 1972; p. 136.

    Google Scholar 

  18. Hahn H.-J., Ziegler B., Butter R., Ziegler M., Henkel M., Fiedler H.: Untersuchungen an Langerhans’schen Inseln in vitro. VII. Vergleichende Untersuchungen zur Stimulation der Insulinsekretion durch unterschiedliche Substrate bei der Ratte — Endokrinologie61, 258, 1973.

    PubMed  CAS  Google Scholar 

  19. Hedeskov C. J., Capito K.: Effect of Starvation on Insulin Secretion and Glucose Metabolism in Mouse Pancreatic Islets — Biochem. J.140, 423, 1974.

    PubMed  CAS  Google Scholar 

  20. Hellman B., Idahl L.-Å., Lernmark Å., Sehlin J., Täljedahl J.-B.: Membrane Sulphydryl Groups and the Pancreatic Beta Cell Recognition of Insulin Secretagogues — In:Malaisse W. H., Pirart J. (Eds): Diabetes. Excerpta Medica/American Elsevier Publ. Co., Amsterdam-New York, 1974; p. 65.

    Google Scholar 

  21. Howell S. L., Green I. C., Montague W.: A Possible Role of Adenylate Cyclase in the Long-Term Dietary Regulation of Insulin Secretion from Rat Islets of Langerhans — Biochem. J.136, 343, 1973.

    PubMed  CAS  Google Scholar 

  22. Idahl L.-Å.: Dynamics of Pancreatic β-Cell Responses to Glucose — Diabetologia9, 403, 1973.

    Article  PubMed  CAS  Google Scholar 

  23. Kipnis D. M.: Studies of Insulin Secretion: Radioimmunoassay of Cyclic Nucleotides and the Role of Cyclic AMP — In:Luft R., Randle P. J. (Eds): On the Pathogenesis of Diabetes Mellitus. Casa Ed. « Il Ponte », Milano, 1970 — Acta diabet. lat.7 (Suppl. 1), 314, 1970.

    Google Scholar 

  24. Kipnis D. M.: Nutrient Regulation of Insulin Secretion in Human Subjects — Diabetes21 (Suppl. 2), 606, 1972.

    Google Scholar 

  25. Köhler P.: Verbesserte Glukoseoxydasemethode zur Blutzuckerbestimmung im Routinebetrieb des klinischen Laboratoriums — Z. Ges. inn. Med.17, 674, 1962.

    Google Scholar 

  26. Kohnert K. D., Hahn H.-J., Zühlke H., Schmidt S., Fiedler H.: Breakdown of Exogenous Insulin by Langerhans Islets of the Pancreas in Vitro — Biochim. biophys. Acta (Amst.)338, 68, 1974.

    CAS  Google Scholar 

  27. Kuo W. N., Hodgins D. S., Kuo J. F.: Regulation by Various Hormones and Agents of Adenosine-3′,5′-Monophosphate Level in Islets of Langerhans of Rats — Biochem. Pharmacol.23, 1387, 1974.

    Article  PubMed  CAS  Google Scholar 

  28. Lin B. J., Nagy B. R., Haist R. E.: Effect of Various Concentrations of Glucose on Insulin Biosynthesis — Endocrinology92, 309, 1972.

    Article  Google Scholar 

  29. Lopez-Quijada C., Gomez-Acebo J., Blazquez E.: Effect of Starvation upon Insulin Secretion in the Rabbit — Acta diabet. lat.6, 820, 1969.

    Article  CAS  Google Scholar 

  30. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J.: Protein Determination with the Folin Phenol Reagent — J. biol. Chem.193, 265, 1951.

    PubMed  CAS  Google Scholar 

  31. Malaisse W. J.: Hormonal and Environmental Modification of Islet Activity — In:Steiner D. F., Freinkel N. (Eds): Handbook of Physiology. Vol. I. Section 7: Endocrine Pancreas. Amer. Physiol. Soc., Washington, 1973; p. 237.

    Google Scholar 

  32. Malaisse W. J., Malaisse-Lagae F., Wright P. H.: Effect of Fasting upon Insulin Secretion in the Rat — Amer. J. Physiol.213, 843, 1967.

    PubMed  CAS  Google Scholar 

  33. Mariani M.-M., Chapal J., Loubatières A. L.: Influence of the Nutritional State upon Insulin Secretion in the Rat — C. R. Soc. Biol. (Paris)166, 155, 1972.

    CAS  Google Scholar 

  34. Matschinsky F. M., Ellerman J. E., Krzanowksi J., Kotler-Brajtburg J., Landgraf R., Fertel R.: The Dual Function of Glucose in Islets of Langerhans — J. biol. Chem.246, 1007, 1971.

    PubMed  CAS  Google Scholar 

  35. Matschinsky F. M., Landgraf R., Ellerman J., Kotler-Brajtburg J.: Glucoreceptor Mechanisms in Islets of Langerhans — Diabetes21 (Suppl. 2), 555, 1972.

    PubMed  CAS  Google Scholar 

  36. Michaelis D., Hahn H.-J., Michael R., Knospe S., Schäfer H., Jutzi E., Wulfert P.: Effekte der intravenösen Nikotinsäureinfusion auf Substrat-, Metabolit- und Hormonkonzentrationen im Blut beim juvenil-manifestierten Diabetes mellitus — Diabetologia6, 550, 1970.

    Article  PubMed  CAS  Google Scholar 

  37. Montague W., Cook J. R.: The Role of Adenosine 3′,5′-Cyclic Monophosphate in the Regulation of Insulin Release by Isolated Rat Islets of Langerhans — Biochem. J.122, 115, 1971.

    PubMed  CAS  Google Scholar 

  38. Panten U., Christians J.: Effects of 2-Endo-Aminonorbornane-2-Carboxylic Acid upon Insulin Secretion and Fluorescence of Reduced Pyridine Nucleotides of Isolated Perifused Pancreatic Islets — Naunyn-Schmiedeberg’s Arch. Pharmak. exp. Path.276, 55, 1973.

    Article  CAS  Google Scholar 

  39. Panten U., Christians J., von Kriegstein E., Poser W., Hasselblatt A.: Effect of Carbohydrates upon Fluorescence of Reduced Pyridine Nucleotides from Perifused Isolated Pancreatic Islets — Diabetologia9, 477, 1973.

    Article  PubMed  CAS  Google Scholar 

  40. Selawry H., Gutman R., Fink G., Recant L.: The Effect of Starvation on Tissue Adenosine 3′,5′-Monophosphate Levels — Biochem. biophys. Res. Commun.51, 198, 1973.

    Article  PubMed  CAS  Google Scholar 

  41. Steinke J., Patel T. N., Ammon H. P. T.: Relationship between Glucose and Tolbutamid Induced Insulin Release and Insulin Content in Single Pancreatic Rat Islets — Metabolism21, 465, 1972.

    Article  PubMed  CAS  Google Scholar 

  42. Turner D. S., Young D. A. B.: The Effect Fasting and Selective Refeeding on Insulin Release in the Rat — Acta endocr. (Kbh.)72, 46, 1973.

    CAS  Google Scholar 

  43. Vance J. E., Buchanan K. D., Williams R. H.: Effect of Starvation and Refeeding on Serum Immunoreactive Glucagon and Insulin Levels — J. Lab. clin. Med.72, 290, 1968.

    PubMed  CAS  Google Scholar 

  44. Voyles N., Gutman R. A., Selawry H., Fink G., Penhos J. C., Recant L.: Interaction of Various Stimulators and Inhibitors on Insulin Secretion in Vitro — Hormone metab. Res.4, 65, 1973.

    CAS  Google Scholar 

  45. Ziegler B., Butter R., Ziegler M., Hahn H.-J., Fiedler H.: Untersuchungen an Langerhans’schen Inseln in vitro. X. Kultivierung Langerhans’scher Inseln der Wistarratte und deren Insulinsekretionsverhalten — Acta biol. med. germ.32, 503, 1974.

    PubMed  CAS  Google Scholar 

  46. Ziegler M., Michael R., Stein H. E., Klatt D.: Radioimmunoassay von Pankreasglukagon isolierter Langerhans’scher Ratteninseln nach dem Prinzip der Rücktitration — Radiobiol. Radiother. (Berl.)15, 79, 1974.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zentralinstitut für Diabetes «Gerhardt Katsch» Karlsburg/Greifswald Bereich für Experimentelle Diabetesforschung, Germany

    Hans-Jürgen Hahn & Heinz Fiedler

Authors
  1. Hans-Jürgen Hahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Heinz Fiedler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hahn, HJ., Fiedler, H. Investigations on isolated islets in vitro. IX. Influence of food deprivation and glucose refeeding in vitro on insulin secretion. Acta diabet. lat 11, 457–467 (1974). https://doi.org/10.1007/BF02624585

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02624585

Key-words

Search

Navigation