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Assays for the Expression and Release of Insulin and Glucose-Regulating Peptide Hormones from Pancreatic β-Cell

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Drug Discovery and Evaluation: Pharmacological Assays

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Abstract

The in vitro perfusion of the isolated rat pancreas as described by Anderson and Long (1947), Ross (1972), Grodsky and coworkers (1983, 1984), and Muñoz and coworkers (1995) offers the advantage to study the influence of carbohydrates, hormones, and drugs such as sulfonylureas not only on insulin but also on glucagon and somatostatin secretion without interference of secondary effects resulting from changes in hepatic, pituitary, or adrenal functions.

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References and Further Reading

Insulin Release from the Isolated Perfused Rat Pancreas; Insulin Release from the Isolated Perifused Rat Pancreatic Islets

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  1. Helmholtz Center Munich, Helmholtz Diabetes Center, Institute for Diabetes and Obesity, Munich, Germany

    Günter Müller

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  1. Dieburg, Germany

    Franz J. Hock

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Müller, G. (2015). Assays for the Expression and Release of Insulin and Glucose-Regulating Peptide Hormones from Pancreatic β-Cell. In: Hock, F. (eds) Drug Discovery and Evaluation: Pharmacological Assays. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27728-3_158-1

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