Summary
Cultured glomerular epithelial cells form a continuous monolayer of polyhedral-shaped cells. PGE2 (1 μg/ml) in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine (MIX) markedly raises intracellular and medium cyclic AMP (cAMP) levels at 20 min (intracellular: MIX alone, 112 ± 6.6 pmol cAMP/mg protein, MIX plus PGE2, 2252±63 pmol cAMP/mg protein; medium: MIX, 20.6±2.1 pmol cAMP/mg protein; MIX plus PGE2, 117±3.8 pmol cAMP/mg protein). By 2 h, when cellular and medium cAMP levels were still elevated, the cells underwent a change in shape that was similar to dome formation (15 to 20% of the monolayer changing shape). Derivatives of cAMP [i. e. dibutyryl and 8-(4-chlorophenylthio)-cAMP], when added to the incubation medium also caused shape change in glomerular epithelial cells at 2 h; cAMP itself did not. The formation of domes has been used as a morphological indicator of the vertorial transport of salt and water in other cultured epithelial cells.
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This work was supported by grant AM 29787 from the National Institutes of Health, Bethesda, MD.
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Kreisberg, J.I., Patel, P.Y., Venkatachalam, M.A. et al. Elevations of intracellular cAMP result in a change in cell shape that resembles dome formation in cultured rat glomerular epithelial cells. In Vitro Cell Dev Biol 22, 392–396 (1986). https://doi.org/10.1007/BF02623528
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DOI: https://doi.org/10.1007/BF02623528