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Establishment of embryogenic suspension cultures of a wild relative of cotton (Gossypium klotzschianum Anderss.)

  • Plant Cellular and Developmental Biology
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Summary

Maintainable, highly embryogenic suspension cultures of a wild relative of cotton (Gossypium klotzschianum Anderss.) have been obtained. Callus with no apparent organization was used to establish the liquid culture. Callus growth conditions as well as suspension medium composition were optimized. A visual selection scheme was beneficial for the maintenance of the embryogenic suspension. These liquid cultures have been maintained for over 10 mo. with no loss in embryogenic capacity. The somatic embryos developed after transfer of the embryogenic tissues to a hormone-free liquid medium.

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Author notes

  1. Ann A. Reilley

    Present address: DNA Plant Technology Corporation, 182 Lewis Road, 95076, Watsonville, CA

Authors and Affiliations

  1. Department of Agronomy, Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH

    John J. Finer

  2. Department of Soil and Crop Sciences, Texas A&M University, 77843, College Station, Texas

    Ann A. Reilley & Roberta H. Smith

Authors
  1. John J. Finer
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  2. Ann A. Reilley
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  3. Roberta H. Smith
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Additional information

Salaries and research support were provided by State and Federal funds appropriated to OSU-OARDC. This is journal article No. 71-87.

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Finer, J.J., Reilley, A.A. & Smith, R.H. Establishment of embryogenic suspension cultures of a wild relative of cotton (Gossypium klotzschianum Anderss.). In Vitro Cell Dev Biol 23, 717–722 (1987). https://doi.org/10.1007/BF02620986

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  • DOI: https://doi.org/10.1007/BF02620986

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