Abstract
Somatic embryoids differentiated in suspension cultures of G. klotzschianum after 3–4 weeks of culture in a liquid medium containing glutamine (optimally, 10–15 mM). Embryogenesis occurred after a preculture of callus on a medium containing 10 mg/l of the cytokinin, 2iP. The embryoids had meristematic regions, a well formed epidermis, and formed roots and vestigial leaves. Asparagine was much less effective than glutamine in promoting embryoid differentiation. The presence of 2,4-D in the medium resulted in increased vigor of the suspension cultures and subsequently in the formation of many embryoids, but does not seem to be necessary for somatic embryogenesis in cotton.
This is a preview of subscription content, log in via an institution to check access.
References
Ammirato, P.V.: Hormonal control of somatic embryo development from cultured cells of caraway. Interactions of abscisic acid, zeatin, and gibberellic acid. Plant Physiol. 59, 579–586 (1977)
Ammirato, P.V., Steward, F.C.: Some effects of the environment on the development of embryos from cultured free cells. Bot. Gaz. 132, 149–158 (1971)
Brown, S., Wetherell, D.F., Dougall, D.K.: The potassium requirement for growth and embryogenesis in wild carrot suspension cultures. Physiol. Plant. 37, 73–79 (1976)
Capdevila, A.M., Dure, L.: Developmental biochemistry of cottonseed embryogenesis and germination. VIII. Free amino acid pool composition during cotyledon development. Plant Physiol. 59, 268–273 (1977)
Dilworth, M.F., Dure, L.: Developmental biochemistry of cotton seed embryogenesis and germination. X. Nitrogen flow from arginine to asparagine in germination. Plant Physiol. 61, 698–702 (1978)
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158 (1968)
Halperin, W.: Morphogenesis in cell cultures. Ann. Rev. Plant Physiol. 20, 395–418 (1969)
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497 (1962)
Price, H.J., Smith, R.H.: Tissue culture of Gossypium species and its potential in cotton genetics and crop improvement. Beltwide Cotton Prod. Res. Conf. Proc., National Cotton Council, Memphis, Tenn., USA, pp. 51–55 1977
Price, H.J., Smith, R.H., Grumbles, R.M.: Callus cultures of six species of cotton (Gossypium L.) on defined media. Plant Sci. Lett. 10, 115–119 (1977)
Reinert, J.: Über die Kontrolle der Morphogenese und die Induktion von Adventivembryonen an Gewebekulturen aus Karotten. Planta 53, 318–338 (1959)
Sass, J.R.: Botanical microtechnique, 3rd edn. Ames: Iowa State Univ. Press 1958
Smith, R.H., Price, H.J., Thaxton, J.B.: Defined conditions for the initiation and growth of cotton callus in vitro. I. Gossypium arboreum. In Vitro 13, 329–334 (1977)
Sondahl, M.R., Sharp, W.R.: High frequency induction of somatic embryos in cultured leaf explants of Coffea arabica L. Z. Pflanzenphysiol. 81, 395–408 (1977)
Steward, F.C., Mapes, M.O., Mears, K.: Growth and organized development of cultured cells. II. Organization in cultures from freely suspended cells. Am. J. Bot. 45, 705–708 (1958)
Stewart, J. McD., Hsu, C.L.: In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137, 113–117 (1977)
Tazawa, M., Reinert, J.: Extracellular and intracellular chemical environments in relation to embryogenesis in vitro. Protoplasma 68, 157–173 (1969)
Vasil, I.K., Hildebrandt, A.C.: Variations of morphogenetic behavior in plant tissue cultures. II. Petroselinum hortense. Am. J. Bot. 53, 869–874 (1966)
Author information
Authors and Affiliations
Additional information
Technical Article 14646 from the Texas Agricultural Experiment Station
Rights and permissions
About this article
Cite this article
Price, H.J., Smith, R.H. Somatic embryogenesis in suspension cultures of Gossypium klotzschianum anderss. Planta 145, 305–307 (1979). https://doi.org/10.1007/BF00454456
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00454456