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Two relative efficiencies of polymorphic enzymes for characterizing cell lines, detecting contaminations, and monitoring transplants

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Summary

A new calculation of the relative efficiency of polymorphic enzyme markers, called the REB, was determined and compared with one of Fisher's determinations of the relative efficiency called REA here. The REA estimates the chance of failing, and 1-REA of succeeding, to show a phenotypic difference between two randomly selected persons or cultured cell lines (Case 1). In this study it was shown that the REA also estimates the chance of detecting a cell line mislabeling or similar mixup (Case 2) and a cell line cross-contamination leading to the complete replacement of an original line by contaminating line (Case 3). The new REB determines the probability of failing, and 1-REB of succeeding, to detect a contamination of an original line by another line leading to their coexistence, or at least a sufficiently long period of transitional coexistence before one overgrows the other. The REA and REB also apply to determining the efficiency of polymorphic markers in detecting donor and recipient cells in tissue transplants.

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Author notes

  1. William C. Wright

    Present address: Department of Immunogenetics, The Lindsley F. Kimball Research Institute of The New York Blood Center, 310 East 67th Street, 10021, New York, NY

Authors and Affiliations

  1. Human Tumor Cell Laboratory, Sloan-Kettering Institute for Cancer Research, 145 Boston Post Road, 10580, Rye, New York

    William C. Wright

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  1. William C. Wright
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This work was developed from the author's involvement in the human tumor cell-line characterization project at Sloan-Kettering Institute and he acknowledges this opportunity and the benefits of his association with Dr. Jørgen Fogh and colleagues in the Human Tumor Cell Laboratory.

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Wright, W.C. Two relative efficiencies of polymorphic enzymes for characterizing cell lines, detecting contaminations, and monitoring transplants. In Vitro 16, 875–883 (1980). https://doi.org/10.1007/BF02619425

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