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Novel Approaches and Technologies in Molecular HLA Typing

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Molecular Typing of Blood Cell Antigens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1310))

Abstract

The invention of the Polymerase Chain Reaction (PCR) has revolutionized molecular biology enabling gene isolation and characterization in hours rather than days. Scientists working in transplant diagnostics have proven to be pioneers in adapting this molecular technique to the clinical needs of histocompatibility testing. This chapter describes a number of novel genotyping technologies which have been used to address the challenges posed by genetic diversity seen in the extensive polymorphism in HLA genes. These novel approaches include single-stranded and duplex conformational analyses, real-time PCR, microarray hybridization, RNA-based sequencing, and the present day Next Generation Sequencing. The chapter concludes with a brief look at a possible next, Next Generation Sequencing system.

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Authors and Affiliations

  1. Leicester General Hospital, University Hospitals Leicester NHS Trust, Gwendolen Road, Leicester, LE5 4PW, UK

    Paul P. J. Dunn

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  1. Paul P. J. Dunn
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Correspondence to Paul P. J. Dunn .

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Editors and Affiliations

  1. Medical Faculty Mannheim, German Red Cross Blood Service Baden-Wurttemberg – Hessen, Institute of Transfusion Medicine and Immunology, Heidelberg University, Mannheim, Germany

    Peter Bugert

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Dunn, P.P.J. (2015). Novel Approaches and Technologies in Molecular HLA Typing. In: Bugert, P. (eds) Molecular Typing of Blood Cell Antigens. Methods in Molecular Biology, vol 1310. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2690-9_18

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  • DOI: https://doi.org/10.1007/978-1-4939-2690-9_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2689-3

  • Online ISBN: 978-1-4939-2690-9

  • eBook Packages: Springer Protocols

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