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Callus formation and differentiation in tissue cultures of normal and cytoplasmic male sterile sorghum, pepper, sunflower, and tobacco

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Summary

The primary goal of this study was to establish callus cultures of selected plant taxa containing both normal (N) and cytoplasmic male-sterile (CMS) lines. A secondary goal was to attempt to differentiate the calli into whole flowering plants. Undifferentiated and organ differentiated calli were produced in specific lines of N and CMS sorghum, pepper, sunflower, and tobacco. Calli from both N and CMS lines of a given species developed similarly and grew well. Even though whole flowering plants have not yet been produced from any of the lines, this approach allows for good production of callus for studies dealing with CMS.

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Authors and Affiliations

  1. Department of Botany and Plant Pathology, Iowa State University, 50010, Ames, Iowa

    Milton A. Rogers, Helen L. Gál & Harry T. Horner Jr.

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  1. Milton A. Rogers
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  2. Helen L. Gál
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  3. Harry T. Horner Jr.
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Additional information

Journal Paper No. J-7741 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 1914.

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Rogers, M.A., Gál, H.L. & Horner, H.T. Callus formation and differentiation in tissue cultures of normal and cytoplasmic male sterile sorghum, pepper, sunflower, and tobacco. In Vitro 9, 463–467 (1974). https://doi.org/10.1007/BF02616000

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