Summary
In vitro growth rates of transformed (crown gall) and nontransformed cultures ofVinca rosea L. were greater at 32°C than at 25°C. The growth of transformed cells was significantly inhibited by kanamycin, neomycin, and chloramphenicol but not by cycloheximide. Nontransformed cells were inhibited by all four antibiotics., The relative growth rates of transformed cultures induced by four different strains, ofAgrobacterium tumefaciens did not correspond to the relative rates of tumor weight increase observed in vivo nor with the relative weights of tumor tissue in, plants 8 weeks after inoculation with the corresponding bacterial strains.
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References
Braun, A. C.. 1958. A physiological, basis for autonomous growth, of the crown-gall tumor cell. Proc. Natl. Acad. Sci. U. S. A. 44: 344–349.
Braun, A. C.. 1947. Thermal studies on the factors responsible for tumor initiation in crown gall. Am. J. Bot. 34: 234–240.
Manasse, R. J., and J. Lipetz. 1971. A simplified method for isolating, bacteria-free crown gall tissue fromVinca, rosea. Can. J. Bot. 49: 1255–1257.
Theis, T. N., A. J. Riker, and O. N. Allen. 1950. The destruction of crown-gall bacteria in periwinkle by high temperature with high humidity. Am. J. Bot. 37: 792–801.
Braun, A. C., and T. Stonier. 1958. Morphology and physiology of plant tumors. Protoplasmatol. Handb. Protoplasmaforsch. 10(5a): 1–93.
Lipetz, J.. 1965. Crown-gall tumorigenesis: Effect of temperature on wound healing and conditioning. Science 149: 865–867.
Lippincott, J. A., and G. T. Heberlein. 1965. The quantitative determination of the infectivity ofAgrobacterium tumefaciens. Am. J. Bot. 52: 856–863.
Lippincott, J. A., and B. B. Lippincott. 1965. Timing of events in crown-gall tumor development on pinto bean leaves. Dev. Biol. 12: 309–327.
Lipetz, J.. 1962. Calcium and the control of lignification in tissue cultures. Am. J. Bot. 49: 460–464.
Schenk, R. U., and A. C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204.
Manasse, R. J.. 1972. A new culture flask for plant tissue suspension cultures. Experientia 28: 723–724.
Heberlein, G. T.. 1970. Enhancement ofAgrobacterium tumefaciens infectivity by chlorpromazine hydrochloride. Infect. Immun. 2: 468–473.
Brown, R.. 1951. The effects of temperature on the durations of the different stages of cell division in the root-tip. J. Exp. Bot. 2: 96–110.
Evans, H. J., and J. R. K. Savage. 1959. The effect of temperature on mitosis and on the action of colchicine in, root meristem cells ofVicia faba. Exp. Cell Res. 18: 51–61.
Bernaerts, M. J., and J. De Ley. 1963. A biochemical test for crown gall bacteria. Nature (Lond.) 197: 406–407.
Braun, A. C., and H. N. Wood. 1962. On the activation of certain, essential biosynthetic systems in cells ofVinca rosea L. Proc. Natl. Acad. Sci. U.S.A. 48: 1776–1782.
Pestka, S.. 1971. Inhibitors, of ribosome functions. Ann. Rev. Microbiol. 25: 487–562.
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Manasse, R.J. Physiological comparisons of transformed (crown gall) and nontransformedVinca rosea L. Cells. In Vitro 9, 434–440 (1974). https://doi.org/10.1007/BF02615995
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DOI: https://doi.org/10.1007/BF02615995