Abstract
Reproductive efficiency of herpes simplex virus type 1 has been determined in several normal and mutant human skin fibroblast lines. The mutant cell lines were derived from individuals diagnosed as having Duchenne muscular dystrophy, a disease thought to involve genetically determined membrane defects. Yields of infectious virus, determined by plaque assay on rabbit kidney cell monolayers, were consistently lower from dystrophic cells than from normal cells. The yield from dystrophic lines was 3–20% of the normal. The time course of production of infectious particles did not appear to vary between dystrophic and normal host cells. Also, the initiation of replication of the viral genome did not appear to be altered in the dystrophic lines. It is proposed that a late maturation function is involved in the lower virus productivity in dystrophic cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Ben-Porat, T., Kaplan, A. S. 1973. Replication—biochemical aspects. pp. 191–203. In: Kaplan, A. S. (ed) The herpesviruses. New York: Academic Press.
Darlington, R. W., Moss, L. H. 1968. Herpesvirus envelopment. Journal of Virology2:48–55.
Duff, R., Rapp, F. 1971. Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2. Journal of Virology8:469–477.
Haines, H., Baerwalk, R. J. 1976. Nuclear membrane changes in herpes simplex virus-infected BHK-21 cells as seen by freeze-fracture. Journal of Virology17:1038–1042.
Howland, J. L. 1974. Abnormal potassium conductance associated with genetic muscular dystrophy. Nature251:724–725.
Howland, J. L., Iyer, S. L. 1977. Erythrocyte lipids in heterozygous carriers of Duchenne muscular dystrophy. Science198:309–310.
Keir, H. M., Hay, J., Morrison, J. M., Subak-Sharpe, H. 1966. Altered properties of deoxyribonucleic acid nucleotidyltransferase after infection of mammalian cells with herpes simplex virus. Nature210:369–371.
Keller, J. M., Spear, P. G., Roizman, B. 1970. Proteins specifying different membrane glycoproteins; Proceedings of the National Academy of Sciences of the United States of America65:865–871.
Kieff, E., Bachenheimer, S. L., Roizman, B. 1971. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. Journal of Virology8:125–132.
Klemperer, H. B., Haynes, G. R., Shedden, W. I. H., Watson, D. H. 1967. A virus-specific thymidine kinase in BHK-21 cells infected with herpes simplex virus. Virology31:120–128.
Russel, W. C., Gold, E., Keir, H. M., Omura, H., Watson, D. H., Wildy, P. 1964. The growth of herpes simplex virus and its nucleic acid. Virology22:103–110.
Schwartz, J., Roizman, B. 1969. Similarities and differences in the development of laboratory strains of freshly isolated strains of herpes simplex virus in HEp-2 cells: Electron microscopy. Journal of Virology4:879–889.
Spear, P. G., Keller, J. M., Roizman, B. 1970. Proteins specified by herpes simplex virus. II. Viral glycoproteins associated with cellular membranes. Journal of Virology5:123–131.
Spring, S., Roizman, B., Schwartz, J. 1968. Herpes simplex virus products in productive and abortive infection. II. Electron microscopic and immunological evidence for failure of virus envelopment as a cause of abortive infection. Journal of Virology2:384–392.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Steinhart, W.L. Herpes simplex virus infection in normal and mutant human fibroblast cultures. Current Microbiology 3, 125–128 (1979). https://doi.org/10.1007/BF02602445
Issue Date:
DOI: https://doi.org/10.1007/BF02602445