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Herpes Simplex Virus Growth, Preparation, and Assay

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Herpes Simplex Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1144))

Abstract

In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.

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References

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Acknowledgment

This work was supported by grants from the University of Ferrara, AIDS National Program and PRIN 2009.

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Authors and Affiliations

  1. Section of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Via Luigi Borsari 46, Ferrara, 44121, Italy

    Peggy Marconi & Roberto Manservigi

Authors
  1. Peggy Marconi
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  2. Roberto Manservigi
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Corresponding author

Correspondence to Peggy Marconi .

Editor information

Editors and Affiliations

  1. Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia

    Russell J. Diefenbach

  2. Institute of Virology, University of Zürich, Zürich, Switzerland

    Cornel Fraefel

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Marconi, P., Manservigi, R. (2014). Herpes Simplex Virus Growth, Preparation, and Assay. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus. Methods in Molecular Biology, vol 1144. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0428-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-0428-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0427-3

  • Online ISBN: 978-1-4939-0428-0

  • eBook Packages: Springer Protocols

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