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Hormonabhängige Enzymverteilung in Geweben

VI. Die Bedeutung der Nebenniere und Hypophyse für die alkalische Nierenphosphatase in Ratten

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Summary

A study is conducted to investigate alkaline phosphatase in the rat kidney under various experimental conditions by means of histochemical methods. The results are as follows:

  1. 1.

    A change in the enzymatic activity is not observed in normal animals if, following adrenalectomy, the animals are substituted with NaCl and have overcome the stress of the operation.

  2. 2.

    After a fictivious operation a decrease in the enzymatic activity is observed, the degree of which is related to the stress of the operation.

  3. 3.

    Following hypophysectomy a marked decrease of enzymatic activity — especially in the marginal areas of the medulla — is observed between the 10th and 30th day after operation.

  4. 4.

    In castrated animals or in animals which in addition have undergone adrenalectomie, an increase of enzymatic activity is observed after the administration of desoxicorticosterons. The effect, however, is not as marked as that of oestradiol.

  5. 5.

    Oestradiol, administered repeatedly, effects a marked increase of the enzymatic activity in adrenalectomized animals. The increase is observed predominantly in the medulla. A similar, but in some cases not as marked, result is observed in hypophysectomized animals.

  6. 6.

    From these findings it is concluded that the effect of oestradiol on the alkaline phosphatase in the rat kidney does not depend on the suprarenal gland or hypophysis.

Zusammenfassung

Mit histochemischen Methoden wurde die alkalische Phosphatase der Rattenniere unter verschiedenen experimentellen Bedingungen untersucht. Folgende Befunde wurden erhoben:

  1. 1.

    In normalen Tieren ist eine Änderung der Enzymaktivität nach Adrenalektomie nicht festzustellen, wenn die Tiere mit Kochsalz substituiert werden und die Operationsbelastung überwunden haben.

  2. 2.

    Scheinoperierte Tiere zeigen je nach dem Grad der erfolgten Belastung einen mehr oder weniger stark ausgeprägten Abfall der Enzymaktivität.

  3. 3.

    Nach Hypophysektomie findet sich zwischen dem 10. und 30. postoperativen Tag eine deutliche bis starke Enzymaktivitätsabnahme, vor allem im Außenstreifen des Marks.

  4. 4.

    Desoxycorticosteron bewirkt in kastrierten oder zusätzlich adrenalektomierten Tieren eine Zunahme der Enzymaktivität. Der Wirkungsgrad reicht aber keineswegs an diejenige von Östradiol heran.

  5. 5.

    Östradiol in kleinen, wiederholt gegebenen Dosen führt in adrenalektomierten Tieren zu einer starken Vermehrung der Enzymaktivität vorzugsweise im Mark. Ein ähnliches Resultat, manchmal weniger stark ausgeprägt, findet sich in hypophysektomierten Tieren.

  6. 6.

    Es ergibt sich daraus, daß die Östradiolwirkung auf die alkalische Phosphatase der Rattenniere nicht der Vermittlung der Nebennieren oder der Hypophyse bedarf.

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Authors and Affiliations

  1. Ludwig Aschoff-Haus, Pathologisches Institut der Universität Freiburg i.Br., Bundesrepublik Deutschland

    Otto v. Deimling, Günter Baumann & Harald Noltenius

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  1. Otto v. Deimling
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  2. Günter Baumann
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  3. Harald Noltenius
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v. Deimling, O., Baumann, G. & Noltenius, H. Hormonabhängige Enzymverteilung in Geweben. Histochemie 6, 277–289 (1966). https://doi.org/10.1007/BF02570672

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