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Hormonabhängige Enzymverteilung in Geweben

II. Histochemische Untersuchungen an Nierenphosphatasen Erwachsener ratten

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Zusammenfassung

Das Verhalten der alkalischen Nierenphosphatase wurde in histochemischen Reaktionen bei weiblichen Ratten unter verschiedenen experimentellen Bedingungen untersucht.

  1. 1.

    Testosterongaben in physiologischer Dosis verändern die Intensität und Verteilung der alkalischen Phosphatase nicht wesentlich.

  2. 2.

    Ovarektomie vermindert hingegen die Intensität der Fermentaktivität erheblich.

  3. 3.

    Dieser Effekt nach Ovarektomie kann durch zusätzliche Testosterongaben nicht mehr verstärkt werden.

  4. 4.

    Intensitätsveränderungen der sauren Phosphatase konnten unter den gleichen Bedingungen nicht festgestellt werden.

  5. 5.

    Die Ergebnisse wurden im Zusammenhang mit früher erhobenen Befunden diskutiert.

Summary

The reaction of alkaline kidney phosphatase in female rats is examined under varied conditions.

  1. 1.

    Testosterone in physiological dose does not essentially alter the intensity and the distribution of alkaline phosphatase.

  2. 2.

    On the other hand the intensity of enzyme activity is significantly diminished by ovarectomy.

  3. 3.

    This aftereffect of ovarectomy cannot be strengthened by supplying testosterone in addition.

  4. 4.

    Variations of intensity of acid phosphatase under the same conditions could not be observed.

  5. 5.

    The findings were discussed in connection with the results mentioned previously.

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Literatur

  • Allegra, G., R. Niutta e G. Pastore: Studi bio-istochimici sulle fosfatasi renali in cani florizinati, dopo carico di vitamine ed ormoni. Arch. de Vecchi Anat. pat. 22, 281–295 (1954).

    Google Scholar 

  • Bunting, H.: Kidney alkaline phosphatase of rats following alloxan induced diabetes and acute hypo- and hyperglycaemia. Proc. Soc. exp. Biol. (N.Y). 67, 370–372 (1948).

    Google Scholar 

  • Chirico, G., L. Vercillo e A. Mannino-Sigillo: Modificazioni della fosfatasi alcalina, a livello dell'intestine e del rene della cavia, indotte dalla somministrazione di alcuni ormoni (ACTH, STH, Doca, cortisone, estratto cortinico). Richerche istochimiche. Arch. Sci. med. 80, 1–12 (1955).

    Google Scholar 

  • Deimling, O. v.: Die Darstellung phosphatfreisetzender Enzyme mittels Schwermetall-Simultan-Methoden. Histochemie 4, 48–55 (1964).

    Google Scholar 

  • -, u. G. Baumann: (In Bearbeitung) (1963).

  • —, O. v. Deiming u. H. Noltenius: Hormonabhängige Enzymverteilung in Geweben. I. Histochemische Untersuchungen über die Geschlechtsunterschiede der alkalischen Nierenphosphatase bei erwachsenen Ratten. Histochemie 3, 500–508 (1964).

    Google Scholar 

  • Dunn, T. B.: Some observations on the normal and pathologic anatomy of the kidney of the mouse. J. nat. Cancer Inst. 9, 285–301 (1949).

    Google Scholar 

  • Durlacher, S. H., D. C. Dorrow, and C. M. Winternitz: The effect of low potassium diet and of desoxycorticosterone acetate upon renal size. Amer. J. Physiol. 136, 346–349 (1942).

    Google Scholar 

  • Eger, W., u. H. F. Geller: Über den Einfluß der Nebenschilddrüse auf die Phosphatasen der Nieren. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. (Leipzig) 218, 222–238 (1953).

    Google Scholar 

  • Farris, E. J., and J. Q. Griffith: The Rat in Laboratory Investigation, p. 310f. New York: Hafner Publ. Co. 1962.

    Google Scholar 

  • Ferreri, E., e A. Peyrot: Ricerche istochimiche e biochimiche su alcuni enzimi del nefrone del tritone crestato (Triturus cristatus carnifex Laur.) in seguito ad ipofisectomia. Z. vergl. Physiol. 46, 102–114 (1962).

    Google Scholar 

  • — Ricerche istochimiche e biochimiche su alcuni enzimi des nefrone des tritone crestato (Triturus cristatus carnifex Laur.) in condizioni normali e sperimentali (Trattamento con tiouracile, gonadectomia). Z. Zellforsch. 56, 470–497 (1962).

    Google Scholar 

  • — Recherches biochimiques et histochimiques sur quelques enzymes du rein du Triturus cristatus carnifex Laur. en conditions expérimentales. Ann. Histochim. 7, 77–82 (1962).

    Google Scholar 

  • Fitch, C. D.: Effect of diet and sex on kidney transaminidase activity in the rat. Proc. Soc. exp. Biol. (N.Y.) 112, 636–639 (1963).

    Google Scholar 

  • Folley, S. J., and A. L. Greenbaum: Effects of adrenalectomy and of treatment with adrenal cortex hormones on the arginase and phosphate levels of lactating rats. Biochem. J. 40, 46–51 (1946).

    Google Scholar 

  • Freudenberger, C. B., and E. I. Hashimoto: A summary of data for the effects of ovariectomy on body growth and organ weights of the young albino rat. Amer. J. Anat. 62, 93–119 (1937).

    Google Scholar 

  • Fumagalli, P.: Le modificazioni dell'attivita fosfatasica alcalina nel rene del ratto dopo surrenectomia. Biol. lat. (Milano) 8, 1306–1321 (1955).

    Google Scholar 

  • György, P., J. Seifter, R. M. Tomarelli, and H. Goldblatt: Influence of dietary factors and sex on the toxicity of carbon tetrachloride in rats. J. exp. Med. 83, 449–462 (1946).

    Google Scholar 

  • Herlant, M., et P. S. Timiras: Etude histologique et histochimique des lésions provoquées par les corticoides au niveau du rein du rat. II. Influence sur les phosphatases alcalines et acides et sur les ribonucléines du rein. Acta anat. (Basel) 14, 65–97 (1952).

    Google Scholar 

  • Humm, J. H., C. D. Kochakian, and M. N. Bartlett: Effect of castration and steroids on the arginase and phosphatases of the organs of the guinea pig. Amer. J. Physiol. 155, 251–254 (1948).

    Google Scholar 

  • Knox, W. E., V. H. Auerbach, and E. C. Lin: Enzymatic and metabolic adaptations in animals. Physiol. Rev. 36, 164–254 (1956).

    Google Scholar 

  • Kochakian, C. D.: The effect of various steroid hormones on the “alkaline” and “acid” phosphatases of the kidney of the mouse. Amer. J. Physiol. 145, 118–122 (1945).

    Google Scholar 

  • — The role of hydrolytic enzymes in some of the metabolic activities of steroid hormones. Recent Progr. Hormone Res. 1, 177–216 (1947).

    Google Scholar 

  • — Histochemical study of alkaline phosphatase of the kidney of the castrated mouse after stimulation with various androgens. Amer. J. Physiol. 152, 257–262 (1948).

    Google Scholar 

  • —, and R. Fox: The effect of castration and testosterone propionate on the “alkaline” and “acid” phosphatases of the kidney, liver and intestine of the mouse. J. biol. Chem. 153, 669–674 (1944).

    Google Scholar 

  • —, and E. Robertson: The effect of androgens and hypophysectomy on arginase and phosphatases of the kidneys and liver of the rat. Arch. Biochem. 29, 114–123 (1950).

    Google Scholar 

  • —, and V. N. Vail: The effect of adrenalectomy, adrenal cortex extract, and adrenal cortex extract plus testosterone propionate on the phosphatases of the rat tissues. J. biol. Chem. 156, 779–780 (1944).

    Google Scholar 

  • Korenchevsky, V., M. Dennison, and A. Kohn-Speyer: Changes produced by testicular hormone in normal and castrated rats. Biochem. J. 27, 557–579 (1933).

    Google Scholar 

  • —, and K. Hall: Correlation between sex hormones, thyroid hormones and desoxycortico-sterone as jugded by their effects on the weights of organs of gonadectomized rats. Biochem. J. 35, 726–735 (1941).

    Google Scholar 

  • Kutscher, W., u. A. Wüst: Nebennierenrinde und alkalische Phosphatase. Hoppe-Seylers Z. physiol. Chem. 273, 235–247 (1942).

    Google Scholar 

  • Longley, J. B., and E. R. Fisher: Alkaline phosphatase and the periodic acid Schiff reaction in the proximal tubule of the vertebrate kidney. A study in segmental differentiation. Anat. Rec. 120, 1–22 (1954).

    Google Scholar 

  • Ludden, J. B., E. Krueger, and I. S. Wright: Effect of testosterone propionate, estradiol benzoate and desoxycorticosterone acetate on the kidney of adult rats. Endocrinology 28, 619–623 (1941).

    Google Scholar 

  • Magrassi, B.: Fosfatase alcalina del siero e degli organi in animali surrenalectomizzati, stiroidati, tireosurrenoprivi e tirossinizzati. Arch. Sci. med. 95, 65–78 (1953).

    Google Scholar 

  • Marsh, J. B., and D. L. Drabkin: Kidney phosphatase in alimentary hyperglycemia and phlorizin glycosuria. A dynamic mechanism for renal threshold for glucose. J. biol. Chem. 168, 61–73 (1947).

    Google Scholar 

  • Palazzi, D.: Studio istochimico del comportamento della fosfatasi alcalina nel rene e nel duodeno des ratto surrenectomizzato e irradiato. Arch. E. Maragliano Pat. Clin. 7, 821–836 (1952).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, Theoretical and Applied. London: Churchill Ltd. 1961.

    Google Scholar 

  • Richterich, R.: Enzymatische Vorgänge bei der Harnbereitung: Biochemie. In: E. Buchborn, K. D. Bock: Diurese und Diuretica, S. 91–120. Berlin-Göttingen-Heidelberg: Springer 1959.

    Google Scholar 

  • Rosenberg, Th., and W. Wilbrand: Enzymatic processes in cell membrane penetration. Int. Rev. Cytol. 1, 65–92 (1952).

    Google Scholar 

  • Rucart, G., et C. Payeur-Villeneuve: Etude histochimique des effets de la thyropara- thyreoidectomie et de l'injection d'extrait parathyroidien sur la phosphatase alcaline rénale du rat. Ann. Histochim. 7, 63–68 (1962).

    Google Scholar 

  • Ruyter, J. H. C., D. B. Kroon, and H. Neumann: Effect of bilateral adrenalectomy on alkaline phosphatase activity in the rat in relation to regressive changes in the nephron. Acta anat. (Basel) 14, 42–53 (1952).

    Google Scholar 

  • Sala, G., A. Amira et C. Cavallero: L'azione del cortisone sulla fosfatasi alcalina renale del ratto. Boll. Soc. ital. Biol. sper. 26, 1501–1503 (1950).

    Google Scholar 

  • Schmidt, L., W. Bernauer u. P. Harnasch: Veränderungen im Hormongehalt der Nebennieren männlicher und weiblicher Meerschweinchen nach Vergiftung mit Diphtherietoxin. Arzneimittel-Forsch. (Drug. Res.) 13, 783–786 (1963).

    Google Scholar 

  • Soulairac, A., P. Desclaux et J. Teysseyre: Étude histochimique de la phosphatase alcaline rénale: la régulation endocrinienne de la réabsorption tabulaire du glucose. Ann. Endocr. (Paris) 10, 536–546 (1949).

    Google Scholar 

  • Tissières, A.: L'influence de la castration du testostérone et de la oestradiol sur les phosphatases du rein chez le rat. Acta anat. (Basel) 5, 235–242 (1948).

    Google Scholar 

  • Vail, V. N., and C. D. Kochakian: The effect of adrenalectomy, adrenal cortical hormones and testosterone propionate plus adrenal cortical extract on the alkaline and acid phosphatases of the liver and kidney of the rat. Amer. J. Physiol. 150, 580–587 (1947).

    Google Scholar 

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Authors and Affiliations

  1. Aus dem Ludwig-Aschoff-Haus, dem Pathologischen Institut der Universität Freiburg i.Br., Deutschland

    Günter Baumann, Otto v. Deimling & Harald Noltenius

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  1. Günter Baumann
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  2. Otto v. Deimling
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  3. Harald Noltenius
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Baumann, G., v. Deimling, O. & Noltenius, H. Hormonabhängige Enzymverteilung in Geweben. Histochemie 4, 150–160 (1964). https://doi.org/10.1007/BF00306156

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