Skip to main content
SpringerLink
Log in

L-Phase variants ofAgromyces ramosus

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Agromyces ramosus, which is a numerically prevalent bacterium in soil, was easily induced into the L-phase by growing it on agar media containing low levels of penicillin or glycine. The L-forms were stable after initial contact with the inducing agent and could not be reverted to the bacterial form by any of the procedures tried. These results are discussed in relation to a possible natural occurrence of L-forms of this bacterium in soil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bae, H. C. andCasida, L. E., Jr. 1973. Responses of indigenous microorganisms to soil incubation as viewed by transmission electron microscopy of cell thin sections.—J. Bacteriol.113: 1462–1473.

    PubMed  CAS  Google Scholar 

  • Bae, H. C., Cota-Robles, E. H. andCasida, L. E., Jr. 1972. Microflora of soil as viewed by transmission electron microscopy.—Appl. Microbiol.23: 637–648.

    PubMed  Google Scholar 

  • Balkwill, D. L. andCasida, L. E., Jr. 1973. Microflora of soil as viewed by freeze-etching.— J. Bacteriol.114: 1319–1327.

    PubMed  CAS  Google Scholar 

  • Bibel, D. J. andLawson, J. W. 1972. Development of streptococcal L-form colonies.—J. Bacteriol.112: 602–610.

    PubMed  CAS  Google Scholar 

  • Casida, L. E., Jr. 1965. Abundant microorganism in soil.—Appl. Microbiol.13: 327–334.

    PubMed  Google Scholar 

  • Casida, L. E., Jr. 1972. Interval scanning photomicrography of microbial cell populations.— Appl. Microbiol.23: 190–192.

    PubMed  Google Scholar 

  • Dienes, L.. 1942. The significance of the large bodies and the development of the L type colonies in bacterial cultures.—J. Bacteriol.44: 37–73.

    PubMed  CAS  Google Scholar 

  • Dienes, L.. 1960. Controversial aspects of the morphology of PPLO.—Ann. New York Acad. Sci.79: 356–368.

    Article  CAS  Google Scholar 

  • Dienes, L.. 1967. Morphology and reproductive processes of the L-forms of bacteria. I. Streptococci and staphylococci.—J. Bacteriol.93: 693–702.

    PubMed  CAS  Google Scholar 

  • Fiedler, F. undKandler, O.. 1973. Die Aminosäuresequenz von 2,4-Diaminobuttersäure enthaltenden Mureinen bei verschiedenen coryneformen Bakterien undAgromyces ramosus. —Arch. Mikrobiol.89: 51–66.

    Article  PubMed  CAS  Google Scholar 

  • Gledhill, W. E. andCasida, L. E., Jr. 1969a. Predominant catalase-negative soil bacteria. I. Streptococcal population indigenous to soil.—Appl. Microbiol.17: 208–213.

    PubMed  CAS  Google Scholar 

  • Gledhill, W. E. andCasida, L. E., Jr. 1969b. Predominant catalase-negative soil bacteria. II. Occurrence and characterization ofActinomyces humiferous sp. N.—Appl. Microbiol.18: 114–121.

    PubMed  CAS  Google Scholar 

  • Gledhill, W. E. andCasida, L. E., Jr. 1969c. Predominant catalase-negative soil bacteria. III.Agromyces, gen. n., microorganisms intermediary toActinomyces andNocardia.—Appl. Microbiol.18: 340–349.

    PubMed  CAS  Google Scholar 

  • Hammes, W., Schleifer, K. H. andKandler, O.. 1973. Mode of action of glycine on the biosynthesis of peptidoglycan.—J. Bacteriol.116: 1029–1053.

    PubMed  CAS  Google Scholar 

  • Hijmans, W., van Boven, C. P. A. andClasener, H. A. L. 1969. Fundamental biology of the L-phase of bacteria, p. 68–143.In L. Hayflick, (ed.), The mycoplasmatales and the L-phase of bacteria.—Appleton-Century-Crofts, New York.

    Google Scholar 

  • Labeda, D. P., Hunt, C. M. andCasida, L. E., Jr. 1974. Plating isolation of various catalase-negative microorganisms from soil.—Appl. Microbiol.27: 432–434.

    PubMed  CAS  Google Scholar 

  • Lawson, J. W. andDouglas, J. T.. 1973. Induction and reversion of the L-form ofNeisseria gonorrhoeae.—Can. J. Microbiol.19: 1145–1151.

    Article  PubMed  CAS  Google Scholar 

  • Liebermeister, K.. 1960. Morphology of the PPLO and L-forms ofProteus.— Ann. New York Acad. Sci.79: 326–343.

    Article  CAS  Google Scholar 

  • Marraro, R. V. Pfister, R. M., Rheins, M. S. andKapetanovic, I. 1971 The morphology of induced wall-defective variants ofStreptococcus faecalis as studied by light and electron microscopy.—Can. J. Microbiol.17: 365–371.

    PubMed  CAS  Google Scholar 

  • Razin, S. andOliver, O. 1961. Morphogenesis of mycoplasma and bacterial L-form colonies. —J. Gen. Microbiol.24: 225–237.

    PubMed  CAS  Google Scholar 

  • Reynolds, E. J. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.—J. Cell Biol.17: 208–212.

    Article  PubMed  CAS  Google Scholar 

  • Ryter, A., Kellenberger, E., Birch-Andersen, A. etMaaløe, O. 1958. Etude au microscope électronique de l'acide désoxyribonucléique. I. Les nucléoides des bactéries en croissance active.—Z. Naturforsch.13b: 597–605.

    CAS  Google Scholar 

  • Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. —J. Ultrastruct. Res.26: 31–43.

    Article  PubMed  CAS  Google Scholar 

  • Young, L. S. andArmstrong, D.. 1969. Induction, colonial morphology, and growth characteristics of the L-form ofStreptococcus liquefaciens.—J. Infect. Dis.120: 281–291.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, The Pennsylvania State University, 16802, University Park, Pennsylvania, USA

    A. H. Horwitz & L. E. Casida Jr.

Authors
  1. A. H. Horwitz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. E. Casida Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This research was authorized for publication as paper No. 4765 in the journal series of the Pennsylvania Agricultural Experiment Station on October 25, 1974.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Horwitz, A.H., Casida, L.E. L-Phase variants ofAgromyces ramosus . Antonie van Leeuwenhoek 41, 153–171 (1975). https://doi.org/10.1007/BF02565047

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02565047

Keywords

Search

Navigation