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Identification of vitamin E-dependent water soluble fluorescent compounds in mouse tissues

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Lipids

Abstract

The present paper describes the identification of two vitamin E-dependent, water soluble fluorescent compounds in mouse tissues. Ultraviolet and fluorescent spectroscopy, derivatization with 1-dimethylamino-naphtalene-5-sulfonyl chloride (dansyl chloride) and cochromatography using high performance liquid chromatography (HPLC) were utilized for the identification of the unknown compounds. The water soluble fluorescent compounds in mouse tissues were identified as tyrosine and tryptophan. The compounds were previously found to increase significantly in vitamin E deficiency in various tissues.

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Abbreviations

Dansyl chloride:

1-dimethyl-naphtalene-5-sulfonyl chloride

HPLC:

high performance liquid chromatography

OSLP:

organic solvent-soluble lipofuscin pigment

TRP:

DL-tryptophan

TYR:

DL-tyrosine

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Authors and Affiliations

  1. Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, 551O8, St. Paul, MN

    John D. Manwaring & A. Saari Csallany

Authors
  1. John D. Manwaring
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  2. A. Saari Csallany
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Manwaring, J.D., Csallany, A.S. Identification of vitamin E-dependent water soluble fluorescent compounds in mouse tissues. Lipids 25, 22–26 (1990). https://doi.org/10.1007/BF02562423

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  • DOI: https://doi.org/10.1007/BF02562423

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