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Production of 9-hydroperoxy-γ-linolenic acid by soybean lipoxygenase in a two-phase system

  • Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

9-Hydroperoxy-γ-linolenic acid (9-GOOH) was produced selectively by soybean lipoxygenase (LG) from γ-linolenic acid (GLA) using a two-phase (borate buffer, pH 6.5/hexane) system at a low temperature (10°C) with some anionic surfactants that showed little inhibitory effect on the enzyme at pH 6.5. The system avoided the inhibitory effect of a higher substrate concentration and of hydroperoxide as well as low substrate solubility in an aqueous system. Not lipoxygenase-2 (LG2) but lipoxygenase-1 (LG1) was indicated to be responsible for the production of 9-GOOH. Among the anionic surfactants examined, acetate and sarcosinate were shown to be suitable, but phosphate was not. Ca2+ increased the 9-GOOH productivity. The LG1 fraction gave the maximum yield of 35% with 0.5 mM Ca2+, ECT-3N (anionic surfactant, acetate) at 10°C and at 4.8 mM GLA in an emulsion.

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Authors and Affiliations

  1. Pharmaceutical Development Laboratory, Meiji Seika Kaisha Ltd., 580 Horikawa-cho, Saiwai-ku, 210, Kawasaki, Japan

    Osamu Hiruta

  2. National Chemical Laboratory for Industry, Tsukuba, 305, Ibaraki, Japan

    Toro Nakahara, Toshihiro Yokochi, Yashushi Kamisaka & Osamu Suzuki

Authors
  1. Osamu Hiruta
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  2. Toro Nakahara
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  3. Toshihiro Yokochi
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  4. Yashushi Kamisaka
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  5. Osamu Suzuki
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Hiruta, O., Nakahara, T., Yokochi, T. et al. Production of 9-hydroperoxy-γ-linolenic acid by soybean lipoxygenase in a two-phase system. J Am Oil Chem Soc 65, 1911–1914 (1988). https://doi.org/10.1007/BF02546005

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  • DOI: https://doi.org/10.1007/BF02546005

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