Abstract
The transesterification of phytosterol and soybean oil was performed using Novozym 435 in supercritical carbon dioxide (SC-CO2). The transesterification reaction was conducted in soybean oil containing 5–25 % phytosterol at 55–95 °C and free-water solvent. The effects of temperature, reaction time, phytosterol concentration, lipase dosage and reaction pressure on the conversion rate of transesterification were investigated. The optimal reaction conditions were the reaction temperature (85 °C), reaction time (1 h), phytosterol concentration (5 %), reaction pressure (8 Mpa) and lipase dosage (1 %). The highest conversion rate of 92 % could be achieved under the optimum conditions. Compared with the method of lipase-catalyzed transesterification of phytosterol and soybean oil at normal pressure, the transesterification in SC-CO2 reduced significantly the reaction temperature and reaction time.
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27 April 2021
A Correction to this paper has been published: https://doi.org/10.1007/s00449-021-02575-x
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Acknowledgments
This work was supported by a grant from the Heilongjiang Provincial Basic Scientific Research Business Foundation: Synthesis of phytosteryl esters by esterification and transesterification in vacuo and without solvent using lipases (No: DADOU2014-2). This work was supported by a grant from the National Natural Science Foundation of China (NSFC): Study on the method of controlling TFAs in oil by orientated hydrogenation and mechanism of molecular reaction under CO2 supercritical state (No: 31271886). This work was also supported by a grant from the Key Laboratory of Soybean Biology in Chinese Ministry of Education Northeast Agricultural University, Harbin, China, 150030. The authors are also grateful to the anonymous referees and the editor for helpful comments on an earlier draft.
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Hu, L., Llibin, S., Li, J. et al. Lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO2 . Bioprocess Biosyst Eng 38, 2343–2347 (2015). https://doi.org/10.1007/s00449-015-1469-5
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DOI: https://doi.org/10.1007/s00449-015-1469-5