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Factors affecting product specificity of peanut lipoxygenase

  • Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

Product specificity of peanut lipoxygenase with linoleic acid as the substrate was determined under different conditions. Extraction solvent, extraction temperature, and reaction temperature only slightly affected the isomeric hydroperoxide ratio. A more pronounced effect was reflected in the total amount of hydroperoxide produced and extracted. CHCℓ3:CH3OH was the most thorough solvent, and extraction temperature of 25 C increased the total amount of hydroperoxides recovered. Lower reaction temperatures produced greater quantities of total hydroperoxides. Oxygen tension and pH value markedly affected changes in the hydroperoxide isomeric ratio and the total amount of hydroperoxides produced. Isomerization of the isolated hydroperoxides occurred very rapidly at 25 C, and a linear isomerization rate was observed at 4 C. At −20 C isolated hydroperoxides isomerized only slightly. Concentration of isolated hydroperoxides affected isomerization of the isomeric hydroperoxides; storing hydroperoxides in dilute solutions was most effective in preventing isomerization.

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Authors and Affiliations

  1. U.S. Department of Agriculture Mid-Atlantic Area, Southern Region, Agricultural Research Service, Department of Botany, North Carolina State University, 27650, Raleigh, North Carolina

    J. A. Singleton, H. E. Pattee & M. S. Nelson

  2. Department of Biological and Agricultural Engineering, North Carolina State University, 27650, Raleigh, North Carolina

    J. A. Singleton, H. E. Pattee & M. S. Nelson

Authors
  1. J. A. Singleton
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  2. H. E. Pattee
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  3. M. S. Nelson
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Additional information

Paper number 5293 of the Journal Series of the North Carolina Agricultural Experiment Station, Raleigh, NC 27650.

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Singleton, J.A., Pattee, H.E. & Nelson, M.S. Factors affecting product specificity of peanut lipoxygenase. J Amer Oil Chem Soc 55, 387–390 (1978). https://doi.org/10.1007/BF02911897

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  • DOI: https://doi.org/10.1007/BF02911897

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