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Influence of testosterone administration on the biosynthesis of unsaturated fatty acids in male and female rats

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Lipids

Abstract

Thein vivo effect of testosterone administration to male or female rats on the biosynthesis of unsaturated fatty acids of liver was studied. Twenty-four hours after injection of testosterone (260 μg/kg), δ9 desaturase activity increased significantly, whereas the activities of δ5 and δ6 desaturases were strongly depressed. These effects were more pronounced in female than in male animals. The fatty acid composition of plasma and liver (homogenates, crude microsomes and cytosol) showed differences between the sexes. In males, the percentage of palmitic acid and the 18∶1/18∶0 ratio were higher whereas the 20∶4(n−6)/18∶2(n−6) ratio was lower than in female rats. The administration of testosterone significantly modified the fatty acid pattern in all fractions studied. Analytical data correlated with alterations in the fatty acid desaturase activities caused by the hormone. It is suggested that the mechanism by which testosterone exerts its effect on unsaturated fatty acid biosynthesis is different than that previously demonstrated by glucocorticoid action. The effects produced by testosterone may be of biological significance in atherosclerosis pathogenesis.

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Abbreviations

GLC:

gas-liquid chromatography

Me:

extracted microsomes

Sp:

supernatant soluble fractions

Te:

testosterone-injected

References

  1. Keys, A. (1970)Circulation 20, 186–187.

    Google Scholar 

  2. Chao, Y., Windler, E.E., Chen, C.G., and Havel, R.J. (1979)J. Biol. Chem. 254, 11360–11366.

    PubMed  CAS  Google Scholar 

  3. Kovanem, P.T., Brown, M.S., and Goldstein, J.I. (1979)J. Biol. Chem. 254, 11367–11373.

    Google Scholar 

  4. Semmens, J., Rouse, I., Bellin, L.J., and Masarei, J.R. (1983)Metabolism 32, 428–432.

    Article  PubMed  CAS  Google Scholar 

  5. Webb, O.L., Laskarzewski, P.M., and Glueck, C.J. (1984)Metabolism 33, 971–975.

    Article  PubMed  CAS  Google Scholar 

  6. Tam, S., Archer, T.K., and Deeley, R.G. (1985)J. Biol. Chem. 260, 1670–1675.

    PubMed  CAS  Google Scholar 

  7. Tam, S., Archer, T.K., and Deeley, R.G. (1986)Proc. Natl. Acad. Sci. USA 83, 3111–3115.

    Article  PubMed  CAS  Google Scholar 

  8. Breckwoldt, M., Neulen, J., Wieacker, P., Geyer, H., and Weiland, H. (1988)Dtch. Med. Wochenschr. 113, 218–220.

    CAS  Google Scholar 

  9. Sammuelson, B. (1981)Prog. Lipid Res. 20, 23–30.

    Article  Google Scholar 

  10. Kingsbury, K.J., Brett, C., Stovold, R., Chapman, A., Anderson, J., and Morgan, D.M. (1974)Postgraduate Med Journal 50, 425–440.

    Article  CAS  Google Scholar 

  11. Brenner, R.R., and Peluffo, R.O. (1966)J. Biol. Chem. 241, 5213–5219.

    PubMed  CAS  Google Scholar 

  12. Bernert, H., and Sprecher, H. (1975)Biochim. Biophys. Acta 398, 354–363.

    PubMed  Google Scholar 

  13. Hassam, A.G., Sinclair, A.J., and Crawford, M.A. (1975)Lipids 10, 417–420.

    Article  PubMed  CAS  Google Scholar 

  14. Sprecher, H., and Lee, C. (1975)Biochim. Biophys. Acta 388, 113–125.

    PubMed  CAS  Google Scholar 

  15. Brenner, R.R. (1981)Prog. Lipid Res. 20, 41–47.

    Article  PubMed  CAS  Google Scholar 

  16. Brenner, R.R. (1987) inRecent Advances in Essential Fatty Acid Research (Das, U.N., ed.) pp. 5–18, PIE Academic Publications, Gandhinagar, India.

    Google Scholar 

  17. Imai, Y. (1979)J. Biochem. (Tokyo) 49, 642–648.

    Google Scholar 

  18. Hagenfeldt, L., Paasikivi, J., and Sjögren, A. (1973)Metabolism 22, 1349–1356.

    Article  Google Scholar 

  19. Esteva, O., Baudet, M.F., Laserrere, M., and Jacotot, B. (1986)Biochim. Biophys. Acta 875, 174–182.

    PubMed  CAS  Google Scholar 

  20. Huang, Y.S., McAdoo, K.R., and Horrobin, D.F. (1987)Nutr. Rep. Int. 36, 1171–1183.

    CAS  Google Scholar 

  21. Baudet, M.F., Esteva, O., Laserre, M., and Jacotot, B. (1986)Clin. Physiol. Biochem. 4, 173–186.

    PubMed  CAS  Google Scholar 

  22. Castuma, J.C., Catalá, A., and Brenner, R.R. (1972)J. Lipid Res. 13, 783–789.

    PubMed  CAS  Google Scholar 

  23. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  24. Catalá, A., Nervi, A.M., and Brenner, R.R. (1975)J. Biol. Chem. 250, 7481–7484.

    PubMed  Google Scholar 

  25. Ninno, R.E., de Torrengo, M.P., Castuma, J.C., and Brenner, R.R. (1974)Biochim. Biophys. Acta 360, 124–133.

    PubMed  CAS  Google Scholar 

  26. Ockner, R.K., Burnett, D.A., Lysenko, N., and Manning, J.A. (1979)J. Clin. Invest. 64, 172–181.

    Article  PubMed  CAS  Google Scholar 

  27. Ockner, R.K., Manning, J.A., Poppenhausen, R.B., and Ho, W.K. (1972)Science 177, 56–58.

    Article  PubMed  CAS  Google Scholar 

  28. Hagve, T., and Christophersen, B.O. (1987)Biochim. Biophys. Acta 917, 333–336.

    PubMed  CAS  Google Scholar 

  29. Paulussen, R.J.A., Jansen, G.P.M., and Veerkamp, J.H. (1986)Biochim. Biophys. Acta 877, 342–349.

    PubMed  CAS  Google Scholar 

  30. Hagve, T., and Christophersen, B.O. (1986)Biochim. Biophys. Acta 875, 655–658.

    PubMed  CAS  Google Scholar 

  31. Hagve, T., and Christophersen, B.O. (1987)Biochim. Biophys. Acta 920, 149–154.

    PubMed  CAS  Google Scholar 

  32. Ambadkar, P.M., and Gangaramani, N.F. (1986)J. Reprod. Biol. Comp. Endocrinol. 6, 64–71.

    CAS  Google Scholar 

  33. Okey, R., Ostwald, R., Shannon, A., and Tinoco, J. (1962)J. Nutr. 76, 353–364.

    PubMed  CAS  Google Scholar 

  34. Lyman, R.L., Shannon, A., and Ostwald, R. (1964)Fed. Proc. 21, 391.

    Google Scholar 

  35. Monse, E.R., Okey, R., and Lyman, R. (1962)Metab. Clin. Exptl. 11, 1113–1124.

    Google Scholar 

  36. Lyman, R.L., Shannon, A., and Ostwald, R. (1964)Fed. Proc. 23, 502.

    Google Scholar 

  37. Aftergood, L., and Alfin-Slater, R.B. (1965)J. Lipid Res. 6, 287–294.

    PubMed  CAS  Google Scholar 

  38. Greenberg, S.M., Calbert, C.E., Savage, E.E., and Deuel, H. (1950)J. Nutr. 41, 473–486.

    PubMed  CAS  Google Scholar 

  39. Ostwald, R., Okey, R., Shannon, A., and Tinoco, J. (1962)J. Nutr. 76, 341–352.

    PubMed  CAS  Google Scholar 

  40. Mohrauer, H., and Holman, R.T. (1963)j. Lipid Res. 4, 151–159.

    Google Scholar 

  41. Ostwald, R., Bouchard, P., Miljanich, P., and Shannon, A. (1966)Biochem. J. 97, 485–499.

    Google Scholar 

  42. Lyman, R.L., Ostwald, R., Bouchard, P., and Shannon, A. (1966)Biochem. J. 98, 438–450.

    PubMed  CAS  Google Scholar 

  43. Ostwald, R., Bouchard, P., Shannon, A., Miljanich, P., and Lyman, R.L. (1966)J. Nutr. 88, 303–314.

    PubMed  CAS  Google Scholar 

  44. Huang, Y.S., and Horrobin, R.F. (1986)Biochem. Arch. 2, 267–277.

    CAS  Google Scholar 

  45. Huang, Y., and Horrobin, D.F. (1987)Proc. Soc. Exptl. Biol. Med. 185, 291–296.

    CAS  Google Scholar 

  46. González, S., Nervi, A.M., Peluffo, R.O., and Brenner, R.R. (1983)Lipids 18, 7–11.

    Article  PubMed  Google Scholar 

  47. Jeffcoat, R., and James, A.T. (1977)Lipids 12, 469–474.

    Article  PubMed  CAS  Google Scholar 

  48. Nervi, A.M., Peluffo, R.O., Brenner, R.R., and Leikin, A.I. (1980)Lipids 15, 263–268.

    Article  PubMed  CAS  Google Scholar 

  49. Marra, C.A., de Alaniz, M.J.T., and Brenner, R.R. (1986)Biochim. Biophys. Acta 879, 388–393.

    PubMed  CAS  Google Scholar 

  50. Marra, C.A., de Alaniz, M.J.T., and Brenner, R.R. (1988)Biochim. Biophys. Acta 958, 93–98.

    PubMed  CAS  Google Scholar 

  51. Marra, C.A., de Alaniz, M.J.T., and Brenner, R.R. (1986)Lipids 21, 212–219.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Facultad de Ciencias Medicas, Instituto de Investigaciones Bioquimicas de la Plata (INIBIOLP), CONICET-UNLP, calles 60 y 120, 1900, La Plata, Argentina

    Carlos A. Marra & Maria J. T. de Alaniz

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  1. Carlos A. Marra
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  2. Maria J. T. de Alaniz
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Marra, C.A., de Alaniz, M.J.T. Influence of testosterone administration on the biosynthesis of unsaturated fatty acids in male and female rats. Lipids 24, 1014–1019 (1989). https://doi.org/10.1007/BF02544071

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  • DOI: https://doi.org/10.1007/BF02544071

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