Abstract
This study was conducted to investigate the effects of deteriorated used frying oil (DUFO) and dietary protein levels upon the hepatic microsomal drugmetabolizing enzyme system. Fresh soybean oil was subjected to a deep-frying process at 205±5°C for four six-hr periods. The resultant DUFO was incorporated into high protein (HU) (27% lactalbumin) or low protein (LU) (8% lactalbumin) test diets at a 15% level. High protein (HF) and low protein (LF) diets containing fresh soybean oil served as the control. Male Long-Evans young rats fed the test diets for eight weeks showed decreased fat absorption and increased red blood cell (RBC) in vitro hemolysis. The activities of hepatic aminopyrine N-demethylase (AD), aniline hydroxylase (AH), NADPH-cytochrome C reductase (NCD), UDP-glucuronyl transferase (UDPGT) and glutathione S-transferase (GST) as well as cytochrome P-450 content were significantly increased in rats fed the HU diet. However, the AD, AH and GST activities, as well as the cytochrome P-450 content of the LU group, were increased to a lesser extent and significantly lower than those of the HU group. Rats fed the LU diet were the only group that showed significantly elevated serum GOT (E.C. 2.6.1.1, glutamate-oxaloacetate transaminase) and GPT (E.C. 2.6.1.2, glutamatepyruvate transaminase) values. Supplementation of 0.3% DL-methionine to the HU diet further increased GST activity. Unexpectedly, rats fed the low protein control diet (LF) also had raised levels of AD, AH and UDPGT activities as well as in vitro RBC hemolysis. It was concluded that rat hepatic microsomal enzymes are induced by dietary DUFO and that the level of induction is influenced by dietary protein level.
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References
Artman, N.R.,Adv. Lipid Res. 7:245 (1969).
Nolen, G.A., J.C. Alexander and N.R. Artman,J. Nutr. 93:337 (1967).
Poling, C.E., E. Eagle, E.E. Rice, A.M.A. Durand and M. Fisher,Lipids 5:128 (1970).
Miller, J., and D.R. Landes,J. Food Sci. 40:545 (1975).
Andia, A.B., and J.C. Street,J. Agric. Food Chem. 23:173 (1975).
Hemans, C., F. Kummerow and E.G. Perkins,J. Nutr. 103:1665 (1973).
Chen, M.H., M.J. Shieh, R.L. Chang and Y.C. Tsai,J. Chinese Nutr. Soc. 8:21 (1983).
Mgbodile, M.U.K., and T.C. Campbell,J. Nutr. 102:53 (1972).
Campbell, T.C., and J.R. Hayes,Fed. Proc. 35:2470 (1976).
Hayes, J.R., M.U.K. Mgbodile, A.H. Merrill Jr., L.S. Nerurkar and T.C. Campbell,J. Nutr. 108:1788 (1978).
Kato, R., T. Oshima and S. Tomizawa,Jpn. J. Pharmacol. 18:356 (1968).
Kato, N., T. Tani and A. Yoshida,J. Nutr. 110:16865 (1980).
Hayes, J.R., M.U.K. Mgbodile and T.C. Campbell,Biochem. Pharmacol. 22:1005 (1973).
Marshall, W.J., and A.E.M. McLean, Ibid.:153 (1969).
Kato, N., and A. Yoshida,Agric. Biol. Chem. 43:191 (1979).
Official and Tentative Methods of The American Oil Chemists’ Society, 3rd edn., edited by E.M. Sallee, AOCS, Champaign, IL, 1971, Methods cd 3a-63 and 8–53.
Michael, J.F., and P.A.T. Swoboda,J. Sci. Fd. Agric. 28:387 (1977).
Horwitt, M.K., C.C. Harvey, G.D. Duncan and W.C. Wilson,Am. J. Clin. Nutr. 4:408 (1956).
Franey, R.J., and E. Amador,Clin. Chim. Acta. 21:255 (1968).
Megraw, R.E., D.E. Dunn and H.G. Biggs,Clin. Chem. 25:273 (1979).
Reitman, S., and S. Frankel,Am. J. Clin. Path. 28:56 (1957).
Fujita, T., and G.J. Mannering,J. Biol. Chem. 248:8150 (1973).
Nash, T.,J. Biochem. 55:416 (1953).
Thurman, R.G., L.A. Reinke, S. Belinsky, R.K. Evans and F.C. Kauffman,Arch. Biochem. Biophys. 209:137 (1981).
Habig, W.H., M.J. Pabst and W.B. Jacoby,J. Biol. Chem. 249:7130 (1974).
William, C.H., and H.J. Kamin, Ibid.:587 (1962).
Omura, T., and R. Sato, Ibid.:2370 (1964).
Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, Ibid.:265 (1951).
Folch, J., M. Lees and G.H. Sloane Stanley, Ibid.:497 (1957).
Abell, L.L., B.B. Levy, B.B. Brodies and F.E. Kendall, Ibid.:357 (1952).
Kim, J., and F.J. Kohout inSPSS: Statistical Package for the Social Sciences, 2nd edn., edited by N.H. Nie, C.H. Hull, J.G. Jenkins, K. Steinbrenner and D.H. Bent, McGraw-Hill Co., New York, 1975, pp. 398–430.
Kanazawa, K., E. Kanazawa and M. Natake,Lipids 20:412 (1985).
Horgan, V.J., J.S.L. Philpot, B.W. Poter and D.B. Roodyn,Biochem. J. 67:551 (1957).
Olcott, H.S., and A. Dolev,Proc. Soc. Exp. Biol. Med. 114:820 (1963).
Findlay, G.M., H.H. Draper and J.G. Bergan,Lipids 5:971 (1970).
Nakatsugawa, K., and T. Kaneda,Yukagaku (J. Jpn. Oil Chem. Soc.) 32:361 (1983).
Nakatsugawa, K., and T. Kaneda, Ibid.:74 (1980).
Bergan, J.G., and H.H. Draper,Lipids 5:977 (1970).
Kaunitz, H., C.A. Slanetz, R.E. Johnson, H.B. Knight, D.H. Saunders and D. Swern,J. Am. Oil Chem. Soc. 33:630 (1956).
Kanazawa, K., G. Danno and M. Natake,J. Nutr. Sci. Vitaminol. 21:373 (1975).
Sumerfield, F.W., and A.L. Tappel,Biochim. Biophys. Acta 740:185 (1983).
Yoshioka, M., and T. Kaneda,Yukagaku (J. Jpn. Oil Chem. Soc.) 23:321 (1974).
Imagawa, T., S. Kasai, K. Matsui and T. Nakamura,J. Biochem. 94:87 (1983).
Oarada, M., T. Miyazawa and T. Kaneda,Lipids 21:150 (1986).
Woodcock, B.G., and G.C. Wood,Biochem. Pharmacol. 20:2703 (1971).
Meister, A.,Science 220:472 (1983).
Reed, D.J., and P.W. Beatty,Rev. Biochem. Toxicol. 2:213 (1980).
Denekes, S.M., S.N. Gershoff and B.L. Fanburg,J. Appl. Physiol.: Respirat. Environ. Exercise Physiol. 54:147 (1983).
Denekes, S.M., B.A. Lunch and B.L. Fanburg,J. Nutr. 115:726 (1985).
Yoshioka, M., and T. Kaneda,Yukagaku (J. Jpn. Oil Chem. Soc.) 23:41 (1974).
Yoshioka, M., K. Tachibana and T. Kaneda, Ibid.:327 (1974).
Nishida, T., H. Tsuchiyama, M. Inoue and F.A. Kummerow,Proc. Soc. Exp. Biol. Med. 105:308 (1960).
Kokatnur, M.G., J.G. Bergan and H.H. Draper, Ibid.:254 (1966).
Bieri, J.G.,Ann. N.Y. Acad. Sci. 203:181 (1972).
Bieri, J.G., and R.P. Evarts,J. Am. Diet. Assoc. 66:134 (1975).
Giani, E., I. Masi and C. Galli,Lipids 20:439 (1985).
Miyazawa, T., A. Nagaoka and T. Kaneda,Agric. Biol. Chem. 47:1333 (1983).
Earnster, L., K. Nordenbrand and S. Orrenius, inLipid Peroxides in Biology and Medicine, edited by K. Yagi, Academic Press, Florida, 1982, pp. 55–79.
Chow, C.K.,Am. J. Clin. Nutr. 37:1066 (1979).
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Huang, CJ., Cheung, NS. & Lu, VR. Effects of deteriorated frying oil and dietary protein levels on liver microsomal enzymes in rats. J Am Oil Chem Soc 65, 1796–1803 (1988). https://doi.org/10.1007/BF02542385
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DOI: https://doi.org/10.1007/BF02542385