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Enteral feeding a structured lipid emulsion containing fish oil prevents the fatty liver of sepsis

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Lipids

Abstract

Fish oils (FO) have been shown to reduce plasma triglycerides (TG). In this study we evaluated whether enteral feeding with a structured lipid emulsion (SLE) containing FO and medium-chain triglycerides (MCT) would prevent the hypertriglyceridemia and fatty infiltration of the liver that develops during sepsis. For five days, male Lewis rats (275–300 g) were fed intragastrically a nutritionally complete diet containing a SLE or a similar diet with a soybean oil emulsion (SOE) in place of the SLE. On the fifth day, sepsis was induced by intravenously injecting 8×107 liveEscherichia coli colonies/100 g b.w.; 24 h later the control SLE, septic SLE, control SOE, and septic SOE rats were sacrificed. Diet, but not treatment, had a significant effect on serum TG and free fatty acids (FFA). Feeding the SLE reduced the plasma FFA of the control and septic rats by more than 50% in comparison to both control and septic rats fed the SOE. Soleus muscle activity of lipoprotein lipase from the septic SLE rats was 44% higher than the control SLE rats. Soleus muscle from the septic SLE rats also had a twofold greater activity of lipoprotein lipase than the septic SOE rats. TG did not accumulate in the livers of the septic rats fed SLE when compared to the control SLE rats and the rats fed the SOE. Livers from the septic rats fed the SLE had a third of the TG that were present in the livers from the septic rats fed the SOE. The rate of incorporation of [14C]oleate into liver lipids was significantly lower in septic rats fed SLE than in those fed the SOE. TG esterification was 70% lower in the septic rats fed SLE rather than the SOE. Our findings suggest that the SLE with FO and MCT has a role in the prevention of the sepsis-associated fatty liver by reducing the biosynthesis of liver TG.

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Abbreviations

CH:

cholesterol

FFA:

free fatty acid(s)

FO:

fish oil

LCT:

long-chain triglycerides

LCFA:

long-chain fatty acids

LPL:

lipoprotein lipase

MCFA:

medium-chain fatty acids

MCT:

medium-chain triglycerides

SLE:

structured lipid emulsion

SOE:

soybean oil emulsion

TG:

triglyceride(s)

References

  1. Finley, R.J. (1985) inProspectives on Sepsis and Septic Shock, (Sibbald, W.J., and Sprung, C.L., eds.) pp. 11–26, SCCM, Fullerton.

    Google Scholar 

  2. Cerra, F.B., Siegel, J.H., Border, J.R., Wiles, J., and McMenamy, R.R. (1979)Surgery 86, 409–422.

    PubMed  CAS  Google Scholar 

  3. Wolfe, R.R., Shaw, J.H.F., and Durkot, M.J. (1985)Am. J. Physiol. 248, (Endocrin. Metab. 11), E732-E740.

    PubMed  CAS  Google Scholar 

  4. Lanza-Jacoby, S., and Tabares, A. (1990)Am. J. Physiol. 258, E676-E685.

    Google Scholar 

  5. Lanza-Jacoby, S., and Rosato, E.L. (1994)Metabolism 43, 691–696.

    Article  PubMed  CAS  Google Scholar 

  6. Gluckian, J.C. (1973)J. Infect. Dis. 127, 1–8.

    Google Scholar 

  7. Canonico, P.G., Ayala, E., and Rill, W.L. (1977)Am. J. Clin. Nutr. 30, 1359–1363.

    PubMed  CAS  Google Scholar 

  8. Harris, W.S., Connor, W.E., and McMurry, M.P. (1983)Metabolism 32, 179–184.

    Article  PubMed  CAS  Google Scholar 

  9. Von Lossonczy, T.O., Ruiter, A., Bronsgeest-Schoute, H.C., van Gent, C.M., and Hermus, R.J. (1978)Am. J. Clin. Nutr. 31, 1340–1346.

    Google Scholar 

  10. Phillipson, B.E., Rothrock, D.W., Connor, W.E., Harris, W.S., and Illingworth, R.D. (1985)N. Engl. J. Med. 312, 1210–1216.b

    Article  PubMed  CAS  Google Scholar 

  11. Wong, S.H., Nestle, P.J., Timble, R.P., Storer, G.B., Illman, R.J., and Topping, D.L. (1984)Biochim. Biophys. Acta 71, 103–109.

    Google Scholar 

  12. Nestle, P.J., Connor, W.E., Reardon, M.F., Connor, S., and Boston, R. (1984)J. Clin. Invest. 74, 82–89.

    Google Scholar 

  13. Nossen, J.O., Rustan, A.L., Gloppestad, S.H., Malbakken, S., and Drevon, G.A. (1986)Biochim. Biophys. Acta 879, 56–65.

    PubMed  CAS  Google Scholar 

  14. Rustan, A.C., Christiansen, E.N., and Drevon, C.A. (1992)Biochem. J. 283, 333–339.

    PubMed  CAS  Google Scholar 

  15. Iritani, N., Inoguchi, K., Endo, M., Fukuda, E., and Morita, M. (1980)Biochim. Biophys. Acta 618, 378–382.

    PubMed  CAS  Google Scholar 

  16. Topping, D.L., Trimble, R.P., and Storer, G.B. (1987)Biochim. Biophys. Acta 927, 423–428.

    Article  PubMed  CAS  Google Scholar 

  17. Willumsen, N., Hexeberg, S., Skorve, J., Lundquist, M., and Berge, R.K. (1993)J. Lipid Res. 34, 13–32.

    PubMed  CAS  Google Scholar 

  18. Parrish, C.C., Pathy, D.A., Parkes, J.G., and Angel, A. (1991)J. Cell. Physiol. 148, 493–502.

    Article  PubMed  CAS  Google Scholar 

  19. Wong, S.H., and Marsh, J.B. (1988)Metabolism 37, 1177–1181.

    Article  PubMed  CAS  Google Scholar 

  20. Zhang, Z.J., Wilcox, H.G., Elam, M.B., Castellani, L.W., and Heimberg, M. (1991)Lipids 26, 504–511.

    Article  PubMed  CAS  Google Scholar 

  21. Herzberg, G.R., and Rogerson, M. (1980)Lipids 24, 351–353.

    Article  Google Scholar 

  22. Baltzell, J.K., Woolens, J.T., and Otto, D. (1991)Lipids 26, 289–294.

    Article  PubMed  CAS  Google Scholar 

  23. Lanza-Jacoby, S., Smythe, C., Phetteplace, H., and Tabares, A. (1992)JPEN 16, 353–358.

    CAS  Google Scholar 

  24. Mascioli, E., Leader, L., Flores, E., Trimbo, S., Bistrian, B., and Blackburn, G. (1988)Lipids 23, 623–625.

    Article  PubMed  CAS  Google Scholar 

  25. Bach, A.C., and Babayan, V.K. (1982)Am. J. Clin. Nutr. 36, 950–962.

    PubMed  CAS  Google Scholar 

  26. Teo, T.C., DeMichele, S.J., Sellack, K.M., Babayan, V.K., Blackburn, G.K., and Bistrian, B.R. (1989)Ann. Surg. 210, 100–107.

    Article  PubMed  CAS  Google Scholar 

  27. Teo, T.C., Selleck, K.M., Wan, J.M.F., Pomposelli, J.J., Babayan, V.K., Blackburn, G.L., and Bistrian, B.R. (1991)Metabolism 40, 1152–1159.

    Article  PubMed  CAS  Google Scholar 

  28. Exton, J.H. (1975)Methods Enzymol. 39, 25–37.

    Article  PubMed  CAS  Google Scholar 

  29. Tripp, R.J., Tabares, A., Wang, H.Z., and Lanza-Jacoby, S. (1993)J. Surg. Res. 55, 465–472.

    Article  PubMed  CAS  Google Scholar 

  30. Hamilton, R.L., Berry, M.N., Williams, M.C., and Severinhhaus, E.M. (1974)J. Lipids Res. 15, 182–187.

    CAS  Google Scholar 

  31. Mitzkat, H.J., and Meyer, V. (1973) inIsolated Liver Perfusion and Its Applications, (Bartosek, I., ed.) pp. 79–86.

  32. Folch, J., Lees, M., and Sloane-Stanley, G.H. (1987)J. Biol. Chem. 226, 497–509.

    Google Scholar 

  33. Nilsson-Ehle, P., and Schotz, M.C. (1976)J. Lipid Res. 17, 536–541.

    PubMed  CAS  Google Scholar 

  34. Wong, S.H., and Nestel, P.J. (1987)Atherosclerosis 64, 139–146.

    Article  PubMed  CAS  Google Scholar 

  35. Lang, C.A., and Davis, R.A. (1990)J. Lipid Res. 31, 2079–2086.

    PubMed  CAS  Google Scholar 

  36. Otto, D.A., Baltzell, J.K., and Wooten, J.T. (1992)Lipids 27, 1013–1017.

    PubMed  CAS  Google Scholar 

  37. Halminski, M.A., Marsh, J.B., and Harrison, E. (1991)J. Nutr. 121, 1554–1561.

    PubMed  CAS  Google Scholar 

  38. Yeo, Y.K., and Holub, B.J. (1990)Lipids 25, 811–814.

    Article  PubMed  CAS  Google Scholar 

  39. Otto, D.A., Tsai, C.E., Baltzell, J.K., and Wooten, J.T. (1991)Biochim. Biophys. Acta 1082, 37–48.

    PubMed  CAS  Google Scholar 

  40. Haug A., and Hostmark, A.T. (1987)J. Nutr. 117, 1011–1017.

    PubMed  CAS  Google Scholar 

  41. Scheig, R. (1968) inMedium Chain Triglycerides (Senior, J.R., ed.), University of Pennsylvania Press, Philadelphia, 1968, pp. 39–40.

    Google Scholar 

  42. Dagnelie, P.C., Rietveld, T., Swart, R., Stijnen, T., and van den Berg, J.W. (1994)Lipids 29, 41–45.

    PubMed  CAS  Google Scholar 

  43. Rustan, A.C., Hustvedt, B.E., and Drevon, C.A. (1993)J. Lipid Res. 34, 1299–1309.

    PubMed  CAS  Google Scholar 

  44. Lutz, O., Lave, T., Frey, A., Meraihi, Z., and Bach, A. (1989)Metabolism 38, 507–513.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Surgery, Jefferson Medical College, 1025 Walnut. St., 19107, Philadelphia, Pennsylvania

    Susan Lanza-Jacoby, Hope Phetteplace & Robert Tripp

Authors
  1. Susan Lanza-Jacoby
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  2. Hope Phetteplace
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  3. Robert Tripp
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Lanza-Jacoby, S., Phetteplace, H. & Tripp, R. Enteral feeding a structured lipid emulsion containing fish oil prevents the fatty liver of sepsis. Lipids 30, 707–712 (1995). https://doi.org/10.1007/BF02537796

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  • DOI: https://doi.org/10.1007/BF02537796

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