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Influence of dietary fish oil on the relative synthesis of triacylglycerol and phospholipids in rat liverin vivo

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Lipids

Abstract

The influence of dietary fish oil containing n−3 polyunsaturated fatty acids on the biosynthesis of triacylglycerol relative to total individual phospholipids was studied in rat liverin vivo. The dietary lipid (10% by weight of diet) was either sunflower oil enriched in linoleic acid (SO group) or MaxEPA fish oil/sunflower oil, 9∶1 by weight (FO group) enriched in eicosapentaenoic acid (EPA, 20∶5n−3) plus docosahexaenoic acid (DHA, 22∶6n−3). After a 3-week feeding period, the triacylglycerol content (in μmmol/g liver) was 44% lower in the FO group relative to the SO animals. Thein vivo incorporation of [3H]glycerol into individual hepatic lipids resulted in triacyl-glycerol/total phospholipid radioactivity ratios of 2.1 and 0.9 for the SO and FO groups, respectively. These results indicate an inhibitory effect of dietary EPA/DHA on triacylglycerol relative to phospholipid synthesis from intermediary 1,2-diacylglycerol in rat liverin vivo. This metabolic alteration was accompanied by a substantially lower amount (in μmol/g liver) of arachidonic acid and higher levels of EPA plus DHA in the triacylglycerol, choline glycerophospholipid (CGP), and ethanolamine glycerophospholipid (EGP) of the FO group. A moderately higher labelling of the EGP from [3H]glycerol was observed in the FO as compared to the SO group (as evidenced by CGP/EGP radioactivity ratios of 1.3∶1 and 1.8∶1, respectively). The present study providesin vivo evidence for a dampening effect of dietary fish oil on the synthesis of liver triacylglycerol relative to phospholipid and a moderate alteration ofde novo synthesis of individual phospholipids.

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Abbreviations

CGP:

choline glycerophospholipids

DG:

diacylglycerol

EGP:

ethanolamine glycerophospholipids

IGP:

inositol glycerophospholipids

PL:

phospholipids

SGP:

serine glycerophospholipids

TG:

triacylglycerol

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Authors and Affiliations

  1. Department of Dairy Sciences, Kyungpook National University, Taegu, Korea

    Young K. Yeo

  2. Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Bruce J. Holub

Authors
  1. Young K. Yeo
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  2. Bruce J. Holub
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Yeo, Y.K., Holub, B.J. Influence of dietary fish oil on the relative synthesis of triacylglycerol and phospholipids in rat liverin vivo . Lipids 25, 811–814 (1990). https://doi.org/10.1007/BF02535902

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  • DOI: https://doi.org/10.1007/BF02535902

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