Abstract
When highly unsaturated fatty acids are added to cell cultures, it can become important to include antioxidants in the culture medium to prevent cytotoxic peroxidation. To find an optimal antioxidant for this purpose, the effect of 50 μM α-tocopherol, γ-tocopherol, α-tocopheryl acetate, α-tocopheryl acid succinate, or α-tocopheryl phosphate, or of 1 μMN,N′-diphenyl-1,4-phenylenediamine, was investigated with respect to the agent's ability to prevent lactate dehydrogenase leakage in long-term rat hepatocyte cultures supplemented with 0.5 mM highly unsaturated fatty acids. Formation of thiobarbituric acid reactive substances in the cultures was also measured. α-Tocopheryl acid succinate was found to be the most effective cytoprotective compound, followed byN,N′-diphenyl-1,4-phenylenediamine, α-tocopherol, γ-tocopherol and α-tocopheryl acetate, and α-tocopheryl phosphate was without effect.
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Abbreviations
- DMSO:
-
dimethylsulfoxide
- DPPD:
-
N,N′-diphenyl-1,4-phenylenediamine
- HUFA:
-
highly unsaturated fatty acids
- LDH:
-
lactate dehydrogenase
- MDA:
-
malondialdehyde
- α-T:
-
α-tocopherol
- γ-T:
-
γ-tocopheryl acetate
- TBARS:
-
thiobarbituric acid reactive substances
- TP:
-
α-tocopheryl phosphate
- TS:
-
α-tocopheryl acid succinate
References
Budowski, P., and Sklan, D. (1989) inThe Role of Fats in Human Nutrition (Vergroesen, A.J., and Crawford, M., eds.) pp. 363–406, Academic Press, London.
Kagan, V.E. (1989)Ann. N.Y. Acad. Sci. 570, 121–135.
Mikkelsen, L., Hansen, H.S., Grunnet, N., and Dich, J. (1993)Biochim. Biophys. Acta 1166, 99–104.
Dich, J., and Grunnet, N. (1990) inMethods in Molecular Biology (Pollard, J.W., and Walker, J.M., eds.) Vol. 5, pp. 161–176, Humana Press, New York.
Fariss, M.W. (1990)Free Radicals Biol. Med. 9, 333–343.
Asakawa, T., and Matsushita, S. (1980)Lipids 15, 137–140.
Bidlack, W.R., and Tappel, A.L. (1973),Lipids 8, 177–182.
Jauregui, H.O., Hayner, N.T., Driscoll, J.L., Williams-Holland, R., Lipsky, M.H., and Galletti, P.M. (1981)In Vitro 17, 1100–1110.
Chow, S.C., Sisfontes, L., Björkhem, I., and Jondal, M. (1989)Lipids 24, 700–704.
Tocher, D.R., and Dick, J.R. (1991)Comp. Biochem. Physiol. 100A, 461–466.
Halliwell, B., and Gutteridge, J.M.C. (1989)Free Radicals in Biology and Medicine, Clarendon Press, Oxford.
Carini, R., Poli, G., Dianzani, M.U., Maddix, S.P., Slater, T.F., and Cheeseman, K.H. (1990)Biochem. Pharmacol. 39, 1597–1601.
Packer, L., Maguire, J.J., Mehlhorn, R.J., Serbinova, E., and Kagan, V. (1989)Biochem. Biophys. Res. Commun. 159, 229–235.
Scott, M.L. (1978) inHandbook of Lipid Research (DeLuca, H.F., ed.) Vol. 2, pp. 133–210, Plenum Press, New York.
Maguire, J.J., Kagan, V., Ackrell, B.A.C., Serbinova, E., and Packer, L. (1992)Arch. Biochem. Biophys. 292, 47–53.
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Mikkelsen, L., Hansen, H.S., Grunnet, N. et al. Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acids. Lipids 29, 369–372 (1994). https://doi.org/10.1007/BF02537192
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DOI: https://doi.org/10.1007/BF02537192