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Suppression of growth in a leukemic T cell line by n−3 and n−6 polyunsaturated fatty acids

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Lipids

Abstract

Proliferation in a lekemic T cell line (Jurkat) was suppressed in a dose dependent manner by n−6 and n−3 polyunsaturated fatty acids (PUFA) added to the culture medium. At high concentrations, PUFA have a cytotoxic effect on Jurkat cells. The inhibitory effect of the PUFA was not due to production of prostaglandins, and lipid peroxidation was only partly responsible. In addition to production of peroxides and aldehydes, lipid peroxidation also reduced the plasmalogen levels in these cells. The antioxidant α-tocopherol blocked lipid peroxidation and restored the plasmalogen levels to normal. α-Tocopherol did not totally restore cell proliferation although the MDA-like products in these cultures (supplemented with PUFA) were reduced to control level. Cultures supplemented with n−6 PUFA seemed to respond better to α-tocopherol than n−3 PUFA. This suggests that n−6 PUFA may exert their growth inhibitory effect predominantly via lipid peroxidation while different mechanisms might be operating for the n−3 PUFA.

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Abbreviations

PUFA:

polyunsaturated fatty acid

LT:

leukotriene

PG:

prostaglandin

ALA:

α-linolenic acid

LA:

linoleic acid

OA:

oleic acid

AA:

arachidonic acid

EPA:

eicosapentaenoic acid

DCHA:

docosahexaenoic acid

MDA:

malondialdehyde

FCS:

fetal calf serum

PBS:

phosphate balance saline

DMA:

dimethyl acetal

BHT:

butyl-hydroxytoluene

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Authors and Affiliations

  1. Department of Immunology, Karolinska Institute, Box 60400, 10401, Stockholm, Sweden

    S. C. Chow & M. Jondal

  2. Department of Clinical Chemistry, Huddinge University Hospital, 10401, Stockholm, Sweden

    L. Sisfontes & I. Björkhem

Authors
  1. S. C. Chow
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  2. L. Sisfontes
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  3. I. Björkhem
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  4. M. Jondal
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Chow, S.C., Sisfontes, L., Björkhem, I. et al. Suppression of growth in a leukemic T cell line by n−3 and n−6 polyunsaturated fatty acids. Lipids 24, 700–704 (1989). https://doi.org/10.1007/BF02535207

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  • DOI: https://doi.org/10.1007/BF02535207

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