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Guinea pig bone marrow cells treated with platelet-activating factor generate factor(s) which affects their DNA synthesis and microbicidal activity

  • PAF and Signal Transduction
  • Published:
Lipids

Abstract

We have previously reported that platelet-activating factor (PAF) induces proliferation and microbicidal activity of guinea pig bone marrow cells. In the present study, we have found that the conditioned medium of PAF- or nonmetabolizable PAF agonist-treated guinea pig bone marrow cells augmented DNA synthesis and induced microbicial activity of bone marrow cells. A PAF specific antagonist, CV-6209, inhibited generation of the active conditioned medium by PAF. Addition of the PAF antagonist only partially suppressed the augmentative effect of the active conditioned medium on DNA synthesis; this is consistent with the fact that, because of the rapid breakdown, no appreciable amount of PAF remained in the conditioned medium of PAF-treated cells. Although mouse bone marrow cells did not respond to PAF unlike guinea pig cells, their DNA synthesis was significantly enhanced by the conditioned medium of PAF-treated guinea pig bone marrow cells. Thus, some newly generated factor(s) distinct from the originally inoculated PAF seemed to modulate the bioactions of PAF on bone marrow cells. An appreciable amount of PAF was produced by calcium ionophore-treated guinea pig bone marrow cells. These findings indicate that PAF synthesized in guinea pig bone marrow cells induces generation in the cells of some factor(s) which affects proliferation or microbicidal activity.

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Abbreviations

BSA:

bovine serum albumin

FCS:

fetal calf serum

PAF:

platelet-activating factor

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Author information

Author notes

  1. Toshiyuki Kato

    Present address: Shionogi Research Laboratories, Shionogi & Co., Ltd., 553, Osaka, Japan

  2. Hidetoshi Hayashi

    Present address: Faculty of Pharmaceutical Sciences, Nagoya City University, 467, Nagoya, Japan

  3. Ryohei Yanoshita

    Present address: Technical R&D Division, Terumo Corporation, 259-01, Kanagawa, Japan

  4. Koichi Ikizawa

    Present address: Biological Research Laboratory, Taiho Pharmaceutical Corporation Ltd., 771-01, Tokushima, Japan

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Ichiro Kudo, Toshiyuki Kato, Hidetoshi Hayashi, Ryohei Yanoshita, Koichi Ikizawa, Hiroko Uda & Keizo Inoue

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  1. Ichiro Kudo
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  2. Toshiyuki Kato
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  3. Hidetoshi Hayashi
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  4. Ryohei Yanoshita
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  5. Koichi Ikizawa
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  6. Hiroko Uda
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  7. Keizo Inoue
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Kudo, I., Kato, T., Hayashi, H. et al. Guinea pig bone marrow cells treated with platelet-activating factor generate factor(s) which affects their DNA synthesis and microbicidal activity. Lipids 26, 1065–1070 (1991). https://doi.org/10.1007/BF02536503

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  • DOI: https://doi.org/10.1007/BF02536503

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